卞留贯 沈建康 罗其中 上海第二医科大学附属仁济医院神经外科 200001 中国神经精神疾病杂志 2000 0 26 4


    关键词：神经鞘瘤；微卫星标记；杂合子丢失 期刊 zgsjjsjbzz 0 225-227 论著与学术交流 fur -->


    

【摘要】 目的 分析神经鞘瘤22号染色体(CHR22)杂合子丢失及其与临床行为之间的关系。方法 选择4个具有高度多态性的微卫星标记物，通过变性聚丙烯酰胺凝胶、银染观察36例神经鞘瘤CHR22杂合子丢失(LOH)频率；以免疫组织化学方法检测Ki-67、PCNA的增殖指数。结果 36例神经鞘瘤中15例发生CHR22杂合子丢失(41.6%)，4个微卫星标记物缺失的频率分别为19.4%(D22S268)、22.2%(D22S264)、19.4%(D22S280)、16.7%(CRYB2);发生CHR22缺失的神经鞘瘤的增殖指数高于无缺失者(P <0.05)。结论 CHR22杂合子丢失是神经鞘瘤发生中的常发事件，缺失的区域包括NF2基因，CHR22杂合子丢失与听神经瘤的临床行为有一定的关系，CHR22杂合子丢失可能是神经鞘瘤增殖的重要因素。

Loss ofheterozygosity for markers on 22 chromosome in sporadic schwannoma

Bian Liuguan Shen Jiankang Luo Qizhong

    ( Department of Neurosurgery, Ren Ji Hospital, Shanghai Second Medical University. 145 ShanDong Road,Shanghai. 200001. Tel: 63260930-2116 )

【 Abstract 】 Objective To analyze the loss ofheterozygoisty for markers on 22 chromosome and its relationship with clinical features. Methods To investigate thefrequency of CHR22 LOH in 36 schwannomas, we selected four highly polymorphicmicrosatellite DNA markers (D22S264,D22S268,D22S280,CRYB2) by denatured polyacrylamidegels and silver staining. The proliferative index of schwannoma was calculated by Ki-67,PCNA immunohistochemistry. Results Fifteen of 36 patients with sporadic schwannomas (41.6%)showed allele loss. The frequency of these markers were 19.4% for D22S268, 22.2% forD22S264, 19.4% for D22S280 and 16.7% for CRYB 2. The proliferative index of schwannomaswith LOH were significantly higher than those without LOH (P < 0.05). In acoustic neuromas, patients with LOH wereyounger at the age of diagnosis, and larger tumor size, had shorter history and highergrowth rate. Conclusions CHR22 LOH was a frequent event in the tumorigenesis ofsporadic schwannoma. The presence of CHR22 LOH was related to clinical features.

    【 Key words 】 SchwannomaMicrosattelite markers Loss of heterozygosity ▲

神经鞘瘤可以散发 ......

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