张开泰 王玉芝 韩本立 张开泰 王玉芝 军事医学科学院二所四室(北京 100850)；韩本立 第三军医大学西南医院肝胆外科 中国普外基础与临床杂志 1999 0 6 5


    关键词：原发性肝癌；浸润；nm23-H1基因 期刊 zgpwjcylczz 0 基础与实验研究 fur -->


    

摘要 为了更深入阐明nm23-H1抑制原发性肝癌转移的作用机理，观察nm23-H1表达状况对肝癌细胞浸润相关因素的影响，本实验采用基因转染手段，将外源nm23-H1全长cDNA导入肝癌细胞并以此观察细胞体外浸润能力、细胞内游离Ca²⁺ 以及N-ras基因mRNA表达的变化。结果： nm23-H1转染组发生侵袭的癌细胞数下降(P<0.05)； N-ras mRNA丰度明显降低(P<0.05)； 细胞内游离Ca²⁺ 浓度增高(P<0.05)。结果提示： nm23-H1影响原发性肝癌细胞浸润与转移可能是通过调节癌细胞内的信息传导而实现的。

A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS

Zhang Kaitai, Wang Yuzhi, Han Benli.

    Institute of Beijing Radiation Medicine, Beijing 100850

Abstract For an advanced elucidation of mechanisms of nm23-H1 suppressive effects on metastasis of primary hepatocellular carcinoma (HCC), it is necessary to investigate the correlation between nm23-H1 expression and relative factors involved in the HCC invasion. In present report, full-length cDNA of nm23-H1 was subcloned into pBKCMV vector and transfected into HCC cell line to observe its effects on invasion, cytosolic free Ca²⁺ andN-ras mRNA expression. The results showed that lower expression of N-ras and higher cytosolic free Ca²⁺ in transfected cell line were detected, while the potential of invasion was depressed. It suggests that the suppressive effects on HCC metastasis might interact with intracellular signal transduction which is essential for stimulating cell invasion.

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