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关键词：脱氧核糖核酸核蛋白体；转录活性；嗜银蛋白；图像分析；大肠癌
摘要 为探讨脱氧核糖核酸核蛋白体(rDNA)转录活性在大肠癌的诊断和鉴别诊断、治疗效果及预后判断的意义，通过细胞培养及银染方法，应用CMIAS-008图像分析系统，对59例大肠癌、20例大肠炎性疾病患者及9例健康者的外周血T淋巴细胞rDNA转录活性进行了检测(以核仁组成区嗜银蛋白银染强度来表达)。结果：大肠癌患者rDNA转录活性明显降低，而在大肠炎性疾病组明显升高，分别与正常对照组比较，差异均有显著性意义(P<0.01)；大肠癌手术及化疗后，T淋巴细胞rDNA转录活性则逐渐升高并接近正常对照组，术后3年内肿瘤复发者该指标则又逐渐下降。结论：T淋巴细胞rDNA转录活性的检测可作为大肠癌与大肠炎性疾病的鉴别指标，同时可作为疗效及监测肿瘤复发的参考指标。
THE DETECTION OF T-LYMPHOCYTES rDNA TRANSCRIPTIONACTIVITY IN PERIPHERAL BLOOD AND CLINICAL SIGNIFICANCE FOR COLORECTAL CARCINOMA PATIENT
Wang Jinsui. Department of Pathology, The People′sHospital of Gansu Province, Lanzhou 730000
Abstract To study the significanceof T-lymphocytes rDNA transcription activity in diagnosis, differential diagnosis,therapeutical effect and evaluation of treatment for colorectal carcinoma, 59 cases ofcolorectal carcinoma, 20 cases of colorectal inflammatory disease and 9 volunteers werechoosen to detect the T-lymphocyte rDNA transcription activity of peripheral bloodT-lymphocyte by cell culture and CMIAS-008 image analysis system of Ag-NOR. Results:T-lymphocytes rDNA transcription activity was decreased obviously in colorectalinflammatory patients. Compared with control group, both group showed markedly statisticaldifference (P<0.01). T-lymphocytes rDNA transcription activity increased gradually tonormal groups after operation and chemical treatment for colorectal carcinoma patients;but it decreased for recurrent patients three years after operation. Conclusions: Thedetection of T-lymphocytes transcription activity can be used as a differential criterionfor colorectal carcinoma and colorectal inflammatory disease, meanwhile it also can beused as a reference criterion for evaluation of treatment and supervision of tumorrecurrence.
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