徐 骁 郑树森 陈 智 梁廷波 张 珉 黄东胜 徐 骁 郑树森 梁廷波 张 珉 黄东胜 浙江医科大学附属第一医院肝胆外科(杭州 310003)；陈 智 浙江医科大学传染病研究所 中国普外基础与临床杂志 1999 0 0 4


    关键词：肝肿瘤；聚合酶链反应；nm23-H1基因 期刊 zgpwjcylczz 0 基础与实验研究 fur -->


    

摘要 为探讨人肝癌中肿瘤转移抑制基因nm23-H1的mRNA表达状况，设计特异性引物，应用逆转录聚合酶链反应(RT-PCR)技术检测分析了20例人肝癌组织及相应癌旁肝组织中nm23-H1基因的mRNA表达。结果：特异性引物能成功扩增nm23-H1基因的几乎整个编码序列； 20例肝癌及癌旁肝组织的RT-PCR结果均为阳性，nm23-H1基因mRNA未发生表达缺失或较大变异现象。由此表明：本实验建立的RT-PCR方法为进一步定量测定肝癌组织中nm23-H1 mRNA表达水平奠定了基础。

    

mRNA EXPRESSION OF nm23-H1 GENE IN HUMAN LIVERTUMOR ASSAYED BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION

Xu Xiao, Zheng Shusen, Chen Zhi, et al.

    Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang Medical University, Hangzhou 310003

Abstract To investigate the mRNAexpression of nm23-H1 gene in human liver tumor. In tumor and corresponding nontumoralliver specimens from 20 patients, nm23-H1 mRNA were examined by reverse transcription-polymerase chain reaction (RT-PCR) method with specific primers. Results:The primers designed in this study could amplified nearly entire coding sequence ofnm23-H1 gene. All the samples showed positive expression of nm23-H1 mRNA, indicating therewas no expression loss or obvious alteration. Conclusions: The achievement of RT-PCRmethod lays foundation for quantitative gaugement of nm23-H1 mRNA in liver tumor.

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