【摘要】 目的 研究壳寡糖对小鼠肿瘤生长和转移的抑制作用以及壳寡糖对培养的巨噬细胞相关的细胞因子表达的影响。 方法 接种肿瘤的小鼠尾静脉注射壳寡糖，观察壳寡糖对肿瘤的生长和抑制作用。用相对定量的逆转录-聚合酶链反应(RT-PCR)技术和酶联免疫吸附实验(ELISA)检测壳寡糖对巨噬细胞相关细胞因子转录和翻译水平的影响。 结果 壳寡糖对S180肉瘤的抑瘤率为35.02%(P<0.001)，对Lewis肺癌的转移灶的影响:实验组(2.1±1.73)，对照组(4.0±2.14)，两组比较，P<0.05。巨噬细胞加入壳寡糖作用24h后，细胞因子的表达明显增强(P<0.01)。 结论 壳寡糖具有良好抑制肿瘤生长和转移的作用。壳寡糖可增强巨噬细胞相关的细胞因子表达。

    【关键词】 壳寡糖;肿瘤;巨噬细胞;细胞因子

    Mechanism of anti-tumor and immune regulation of oligochitosan

    WU Hai-ming，HUAXiao-yang，HE Deng-quan.

    Department of General Surgery，Traditional Chinese Medicine Hospital of Yiwu，Yiwu322000，China

    【Abstract】 Objective To study the influence of oligochitosan in inhibiting growth and metastasis of tumor in rats，and in expression of cytokines associated with macrophage.Methods Inhibition of growth of tumor was observed after oligochitosan injected into rats through tail vein，the influence in transcription and translation of cytokines associated with macrophage was measured by RT-PCR and ELISA techniques.Results Inhibition rate of sarcoma S180was35.02%(P<0.001)after injection of oligochitosan.The influence in metastasis of Lewis lung cancer(P<0.05):experiment group(2.1±1.73) ......