【摘要】 目的 了解WT1基因(Wilms’tumor gene)与急性白血病缓解后微小残留病灶的关系。方法 应用逆转录-聚合酶链反应(RT-PCR)方法检测了急性白血病(AL)及慢性粒细胞白血病(CML)中WT1基因不同剪接体的表达情况。结果 AL中WT1基因阳性表达率为50%， CML中WT1基因阳性表达率为40%，正常人无WT1基因表达，WT1基因4种剪接体的表达水平相近。结论 WT1基因在白血病中呈较高表达，其结果可作为白血病残留病灶检测的指标。

    【关键词】 急性白血病；WT1基因；微小残留病灶

    Molecular biological measurement of minimal residual disease in patients with acute leukemia after complete remission

    CHEN Yan，LI Jing-lan.

    Department of Internal Medicine，Huanhu Hospital of Tianjin,Tianjin 300060，China

    【Abstract】 Objective Expression of the Wilms’tumor gene(WT1)was studied in patients with acute leukemia after complete remission.Methods By using reverse transcription polymerase chain reaction (RT-PCR),the expression of WT1 gene in patients with acute leukemia and chronic myeloid leukemia were detected.Results The positive expression rate of WT1 gene was 50.0% in patients with acute leukemia(AL).The positive expression rate of WT1 gene was 40.0% in patients with chronic myeloid leukemia(CML).WT1 gene was not detected in normal control.The positive rate of WT1 gene’s splice variant in AL were different,but these had not significantly different each other.Conclusion WT1 gene is frequently expressed in leukemia,it would be a useful marker for the detection of minimal residual disease(MRD) in leukemia. ......