【摘要】 目的 研究人参皂苷Rb1对体外培养雪旺细胞增殖分化的影响，探讨其促进神经再生的作用和机制。 方法 选用8个月月龄的新西兰兔的坐骨神经体外培养第2代，加入不同浓度的人参皂苷Rb1，继续培养10天，相差显微镜观察计数，绘制各自的增殖曲线;在36h和72h对各组细胞进行流式细胞分析。 结果 活细胞计数含20μg/ml人参皂苷Rb1组的倍增时间为5.3天，明显优于对照组(P<0.01);用含20μg/ml人参皂苷Rb1培养36h、72h后雪旺细胞增殖的流式细胞仪分析，处于S期的雪旺细胞较对照组明显增高(P<0.01)。 结论 人参皂苷Rb1有促进体外培养雪旺细胞快速增殖分化的作用，从而为促进神经损伤的再生途径提供一些新的药物使用基础研究。

    【关键词】 人参皂苷Rb1;雪旺细胞;细胞增殖

    Effects of GinsenosideRb1on the cultured schwann cells in vitro

    YANG Lei，LIU Li-jun，XIAO Jian-de，et al.

    The Second People's Hospital，Shenzhen518035，China

    【Abstract】 Objective To study the effects of Ginsenoside Rb1on the proliferation and differentiation of Schwann's cells and discuss the mechanism of nerve regeneration induced by Ginsenoside Rb1.Methods The sciatic nerve of New zealand rabbit of8months old was cultured in vitro and the second generation was used.Ginsenoside Rb1with different concentrations were dropped into the culture medium for10days.Numbers of cells were counted by contrast microscope and proliferation curves were drawn individually.Furthermore ......