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天麻脂酶同工酶研究
http://www.100md.com 《中华医药杂志》 2005年第6期
天麻,天麻;脂酶;同工酶;电泳,1材料与方法,2结果与分析,3讨论,【参考文献】
     【摘要】 采用聚丙烯酰胺凝胶电泳(PAGE)对3种天麻菌株脂酶(EST)同工酶进行了研究。结果表明,天麻不同菌株之间脂酶同工酶活性不同,谱条带数在4~5条之间,且具有特征谱带。B、G的酶谱条带数基本相同;R、B、G的酶谱条数依次分别为4条,5条,4条;其中基本酶带有3条;G号菌株与B号菌株的相似度指数为0.89,说明这两个菌株间亲缘关系最近。G号菌株与R号菌株的相似度指数为0.75,反映它们之间的亲缘关系相对较远。

    【关键词】 天麻;脂酶;同工酶;电泳

    Study on esterase isozyme of gastrodia elata

    DIANG Rui,JIANG Hai.

    Shanxi Key Laboratory of Bio-resources,Hanzhong723000,China

    【Abstract】 The esterase isozyme(EST)from the three strains of gastrodia elata was studied by using PAGE.The re-sults showed they had the different activity of the sterase isozyme of gastrodia elata.,and had it's peculiar pattern bands,the number of isozyme bands was4~5;the fundamental bands were three.Gastrodia elata Bl.f.glauca S.Chow and gastrodia elata Bl.f.elata specieses'relations are very near;and gastrodia elata Bl.f.viridis Makino'Index of similarity is0.75 ......

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