低氧对胰腺癌细胞株PC-3中IAP-2表达的影响机制
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赵 秋，谷 华，杜 静，覃 华，刘南植
低氧;胰腺癌;凋亡抑制蛋白2;缺氧诱导因子1,赵秋，谷华，杜静，覃华，刘南植，,赵秋，,通讯作者：,Effectofhypoxiaonapopto
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赵秋，谷华，杜静，覃华，刘南植，华中科技大学同济医学院附属同济医院消化内科 湖北省武汉市 430030
赵秋，男，1963-09-01生，湖北省黄冈市人，汉族,1985年同济医科大学本科毕业，1988年同济医科大学硕士，副主任医师，主要从事消化系胰腺病学研究.
通讯作者：赵秋，430030，湖北省武汉市汉口解放大道1095号，华中科技大学同济医学院附属同济医院消化内科. tongji461@sina.com
电话：027-83663611
收稿日期：2005-06-28 接受日期：2005-07-08
Effect of hypoxia on apoptosis inhibitory protein 2 expression and its mechanism in pancreatic cancer cell line PC-3
Qiu Zhao, Hua Gu, Jing Du, Hua Qin, Nan-Zhi Liu
Qiu Zhao, Hua Gu, Jing Du, Hua Qin, Nan-Zhi Liu, Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Qiu Zhao, Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. tongji461@sina.com
Received: 2005-06-28 Accepted: 2005-07-08
Abstract
AIM: To investigate the expression of apoptosis inhibitory protein 2 (IAP-2) in pancreatic cancer cell PC-3 under severe hypoxia, and to explore its relation with hypoxia inducible factor 1(HIF-1).
METHODS: PC-3 cells were cultured under different conditions as follows: normoxia; 20mL/L O2, 50mL/L CO2, and 930mL/LN2 for 4 h (hypoxia); 950mL/L N2 and 50mL/L CO2 for 1, 3, 5 h, respectively (severe hypoxia); reoxygenation after 1 h of severe hypoxia; normoxia with colalt chloride (300 µmol/L). Immunocytochemistry was used to qualitatively evaluate the expression of IAP-2 protein. After extraction of cytoplasmic and nuclear proteins, Western blot was used to quantitatively determine the expression of IAP-2 protein, which was compared with the expression of HIF-1 protein. Then the expression of IAP-2 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: IAP-2 protein was positively expressed in the cytoplasm of PC-3 cells ......
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