腹腔冲洗液和腹膜组织检测胃癌腹腔微转移的临床意义
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王夫景, 高 岩, 黄跃南, 佟佰峰, 张秀云,
RT-PCR;CEA;胃癌;腹膜转移;腹腔冲洗液;细胞学检查,王夫景，黄跃南，佟佰峰，杨维良，,高岩，,张秀云，,通讯作者：,Clinicalsign
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王夫景，黄跃南，佟佰峰，杨维良，哈尔滨医科大学附属第二医院普外科 黑龙江省哈尔滨市 150086
高岩，哈尔滨医科大学附属第二医院普外科 黑龙江省哈尔滨市150086
张秀云，哈尔滨医科大学附属第二医院科研中心 黑龙江省哈尔滨市 150086
黑龙江省十五科技攻关资金资助项目，No. GC02C140-02
通讯作者：高岩，150086，黑龙江省哈尔滨市，哈尔滨医科大学附属第二医院普外科.
收稿日期：2005-06-28 接受日期：2005-07-08
Clinical significance of free cancer cells in peritoneal washes and tissues of patients with gastric cancer
Fu-Jing Wang, Yan Gao, Yue-Nan Huang, Bai-Feng Tong, Xiu-Yun Zhang, Wei-Liang Yang
Fu-Jing Wang, Yue-Nan Huang, Bai-Feng Tong, Wei-Liang Yang, Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Yan Gao, Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Xiu-Yun Zhang, Research Center of the Second Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by the Tenth Five-Year Program of Science and Technology of Heilongjiang Province, No. GC02C140-02
Correspondence to: Yan Gao, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Received: 2005-06-28 Accepted: 2005-07-08
Abstract
AIM: To detect the free cancer cells in the peritoneal washes and tissues, and to explore their significances in the prediction of peritoneal metastasis of gastric cancer.
METHODS: The peritoneal washes and tissues were collected during laparotomy from 38 patients with gastric cancer and 5 with benign gastric disease. Peritoneal lavage cytology was performed for the washes, and the carcinoembryonic antigen (CEA) mRNA of the free cancer cells was detected by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: The CEA mRNA positive rates of the free cancer cells in the peritoneal washes and tissues were 36 ......
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