经兔肝动脉灌注平阳霉素碘油乳剂制作肝纤维化模型
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李 枫, 徐 克, 刘瑞宝
平阳霉素;碘油;肝纤维化;模型,李枫，徐克，刘瑞宝，,李枫，,通讯作者：,Anewmodelofhepaticfibrosisinducedbyintr
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李枫，徐克，刘瑞宝，中国医科大学附属第一医院放射科 辽宁省沈阳市 110001
李枫，男，1973-5-16生，辽宁省大连市人，汉族，中国医科大学2000级硕士研究生，现工作于大连医科大学附属第一医院介入科，主治医师，主要从事肝胆疾病的介入治疗研究.
通讯作者：徐克，110001，辽宁省沈阳市和平区北二马路92号，中国医科大学附属第一医院放射科. xuke@cmuimaging.com
电话：024-23252365
收稿日期：2005-06-28 接受日期：2005-07-09
A new model of hepatic fibrosis induced by intra-arterial injection of iodized oil and pingyangmycin emulsions
Feng Li, Ke Xu, Rui-Bao Liu
Feng Li, Ke Xu, Rui-Bao Liu, Department of Radiology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Dr. Ke Xu, Department of Radiology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. xuke@cmuimaging.com
Received: 2005-06-28 Accepted: 2005-07-09
Abstract
AIM: To investigate the feasibility of setting up a hepatic fibrosis model by intra-arterial injection of emulsions of iodized oil and pingyangmycin.
METHODS: Twenty-four Japanese big-ear rabbits were divided into sham-operation, experiment A and B group according to the doses of emulsions of iodized oil and pingyangmycin (1.2 and 2.0 mg for A and B, respectively) injected into the hepatic artery. The live specimens were obtained 1, 2, 4, 6, 10, and 14 wk after injection. The histological changes were determined by HE staining, and the contents of platelet derived growth factor-B (PDGF-B) were detected by immunohistochemical staining.
RESULTS: Six weeks after injection, typical features of fibrosis appeared in all the liver tissues of group A and B, and the pseudolobules formed in some tissues. In group B, sclerosis with a diameter of 1.0 cm was found in one of the four rabbits at 10th week under light microscope. The contents of PDGF-B were significantly increased in A and B group as compared with those in sham-operation group 1, 2, 4, and 6 wk after injection (integral optical density: 118714±14941, 154344±25102 vs 42745±871, P <0 ......
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