5-氮杂-2'-脱氧胞苷对SW48结肠腺癌细胞生物学行为的影响
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李秀梅，刘南植，倪 志，张 庆，洪 玮
5-氮杂-2'-脱氧胞苷;SW48结肠腺癌细胞;细胞周期;细胞凋亡,李秀梅，刘南植，倪志，张庆，洪玮，,李秀梅，,通讯作者：,Effec
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李秀梅，刘南植，倪志，张庆，洪玮，华中科技大学同剂医学院附属同剂医院消化内科 湖北省武汉市 430030
李秀梅，女，1976年生，湖北襄樊枣阳人，汉族，华中科技大学同济医学院附属同济医院2003级硕士，医师，主要从事消化系统疾病的基础研究.
通讯作者：刘南植，430030，湖北武汉市汉口解放大道1095号，华中科技大学同剂医学院附属同剂医院消化内科. lisalixiumei@126.com
电话：027-62134592
收稿日期：2005-07-15 接受日期：2005-08-03
Effect of demethylating agent 5-Aza-2'-deoxycytidine on human colonic carcinoma cell line SW48
Xiu-Mei Li, Nan-Zhi Liu, Zhi Ni, Qing Zhang, Wei Hong
Xiu-Mei Li, Nan-Zhi Liu, Zhi Ni, Qing Zhang, Wei Hong, Department of Gastroenterology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Nan-Zhi Liu, Department of Gastroenterology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. lisalixiumei@126.com
Received: 2005-07-15 Accepted: 2005-08-03
Abstract
AIM: To explore the effect of demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-CdR) on the growth of human colonic carcinoma cell line SW48, and to investigate the possibility of its application in clinical treatment.
METHODS: Human colonic carcinoma SW48 cells were treated with 0.4, 1.6, 6.4, 25.8, and 102.4 µmol/L 5-Aza-CdR, respectively. Then the growth of the cells was observed by MTT assay. The cell cycle and apoptosis were analyzed by flow cytometry. The expression of the tumor suppressor gene RASSF1A mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS: 5-Aza-CdR inhibited the proliferation of SW48 cells in a time- and concentration-dependent manner (1-5 d, 0.4-102.4 µmol/L). After 5-Aza-CdR treatment, the number of G0/G1 cells was increased, and 5-Aza-CdR blocked the cell cycle at G1 phase. The apoptotic rate was also increased significantly. RASSF1A gene was reactivated by 5-Aza-CdR in SW48 cells not expressing RASSF1A ......
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