摘要:目的 了解特定人群DNA氧化损伤情况，比较肉类烧烤师、煎炸烹调师、面点师和一般服务员尿中8-羟基脱氧鸟苷(8-OHdG)水平。 方法 用8-OHdG的酶联免疫试剂盒检测尿中8-OHdG水平，用苦味酸法测定尿中的肌酐，计算8-OHdG的肌酐校正值。 结果 肉类烧烤师、煎炸烹调师、面点师、一般服务员尿中8-OHdG水平分别为(16.24±3.56)ng/mgCr、(17.53±4.28)ng/mgCr、(11.65±2.45)ng/mgCr、(12.38±2.12)ng/mgCr。显示肉类烧烤师和煎炸烹调师的尿中8-OHdG水平高于面点师和一般服务员。且前二者的8-OHdG水平与其工龄有关。 结论 饮食业肉类烧烤师和煎炸烹调师尿中8-OHdG较高，应加强相应的职业防护。

    关键词:尿8-羟基脱氧鸟苷;烹调;DNA氧化损伤

    Study on the damage to oxidative DNA by determination of8-OHdG level in the urine of four kinds of cuisine cooks.

    KE Yue-bin，XU Yuan-yuan，TAN Chun-rong，et al.(Shenzhen Municipal Center for Disease Control and Prevention，Shen-zhen518020，Guangdong，P.R.China)

    Abstract:Objective To understand the damage to oxidative DNA by determination of8-OHdG level in the urine of meat bar-becue cooks，fry cooks，desserts cooks and food waiters. Methods ELISA kits for8-hydroxy-2'-deoxyguanosine(8-O-HdG)check are used to detect8-OHdG in urine samples.Picrate method is used for creatine detection.8-OHdG level is adjusted by creatine in urine. Results The results of8-OHdG levels in urine are as follows:16.24±3.56ng/mgCr for barbecue cooks ......