[摘要] 目的：建立纯化原代培养的小鼠胚胎腭突间充质细胞的纯化方法。方法：应用Dispase酶4 ℃消化处理离体小鼠胚胎腭突18 h，通过振荡法使上皮和间充质分离，吸去上皮片。结果：应用流式细胞仪检测，胚胎腭突间充质细胞内几乎不含上皮细胞。结论：本方法为体外研究提供了理想的模型。

    [关键词] 胚胎 腭间充质细胞 细胞培养 纯化

    Purification of Cultured C57BL/6J Mouse Embryonic Palatal mesenchymal Cells in Vitro.

    XIAO Wen-lin, SHI Bing, LI Sheng, et al.

    West China College of Stomatology, Sichuan University， Chengdu 610041

    [Abstract]Objective: To establish an effective purification method for primary culture of mouse embryonic palatal mesenchymal(EPM) cells. Methods: The dissected embryonic palatal shelves were incubated with Dispase at 4℃ for 18 hours, and then the organ rudiments were agitated, a small-bore pipette was used to separate the loosened epithelium from mesenchymal. Results: The EPM cells contained hardly epithelial cells by flow cytometry analysis. Conclusion: This method system provides a valuable model for studies of EPM cells in vitro.

    [Key words] Embryo Palatal mesenchymal cell Cell culture Purification

    体外培养的细胞源于动物机体 ......