塞来昔布对胃癌细胞生长及 ERK2 表达的影响
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张 勇，蒋明德，曾维政，徐 辉，熊碧君，翁 敏
塞来昔布;细胞外信号调节激酶2;胃癌细胞株SGC790,塞来昔布对胃癌细胞生长及ERK2表达的影响,张勇,蒋明德,曾维政,徐辉,熊碧君,翁敏，,张勇,通讯作者：,电话：,收稿日期：,接受日期：,Ef
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张勇, 蒋明德, 曾维政, 徐辉, 熊碧君, 翁敏，中国人民解放军成都军区总医院消化内科 四川省成都市 610083
张勇, 男, 1967-12-13生, 重庆市壁山县人, 汉族, 1995年第三军医大学硕士, 副主任医师. 发表文章30篇, 主要从事功能性胃肠疾病及胃癌的基础与临床研究.
通讯作者：张勇, 610083, 四川省成都市, 中国人民解放军成都军区总医院消化内科. zhangyo@163.com
电话：028-86570349
收稿日期：2005-07-19 接受日期：2005-07-28
Effects of celecoxib on cellproliferation and ERK2 expression of ERK2 in gastric cancer cells
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui-Xu, Bi-Jun Xiong, Min-Weng
Yong Zhang, Ming-De Jiang, Wei-Zheng Zeng, Hui-Xu, Bi-Jun Xiong, Min-Weng, Department of Gastroenterology, General Hospital, Chengdu Military Command of Chinese PLA, Chengdu 610083, Sichuan Province, China
Correspondence to: Yong-Zhang, Department of Gastroenterology, General Hospital, Chengdu Military Command of Chinese PLA, Chengdu 610083, Sichuan Province, China. zhangyo@163.com
Received: 2005-07-19 Accepted: 2005-07-28
Abstract
AIM: To explore the role of celecoxib, a selective COX-2 inhibitor, in the proliferation of human gastric carcinoma cells SGC7901, and to investigate the relationship between cell proliferation and the expression of extracellular signal-regulated kinase 2 (ERK2).
METHODS: The cell proliferation of SGC7901 cells was measured by MTT assay, and the apoptoticsis rate of the cells was examined by TUNEL staining method and flow cytometry. The expression of phosphated ERK2 was detected by immunohistochemistry and quantified measured by image analysis systems.
RESULTS: Celecoxib inhibited the proliferation of SGC7901 gastric carcinoma cells and the expression of phosphated ERK2, as well as induced the apoptosis of the SGC-7901 cells in a dose-dependent manner. The growth inhibitory rates of SGC-7901 cells treated with 10, 20, 40, 80 and, 160 μmol/L celecoxib for 48 h were 9.8%, 30%, 58.9%, 76.3%, and 88.3%, respectively. The SGC7901 cells presented typical morphological features of apoptosis by TUNEL staining ......
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