sp600125 对乙醛刺激的大鼠肝星状细胞凋亡及 Caspase-3 蛋白表达的影响
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唐 文, 蒋明德, 李小安
肝纤维化;肝星状细胞;sp600125;Caspase-3,sp600125对乙醛刺激的大鼠肝星状细胞凋亡及Caspase-3蛋白表达的影响,唐文,蒋明德,李小安,通讯作者：,电话:,传真:,收稿日
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唐文, 蒋明德, 李小安, 第三军医大学成都临床学院 成都军区总医院消化内科 四川省成都市 610083
全军十五医药卫生科研基金项目, No. 01MB037
通讯作者：蒋明德, 610083, 四川省成都市成都军区总医院消化内科. jiangmd88@yahoo.com.cn
电话: 028-86570346 传真: 028-86570346
收稿日期：2005-07-19 接受日期：2005-08-05
Effects of sp600125 on acetaldehyde-induced apoptosis of hepatic stellate cells and expression of Caspase-3 protein in rats
Wen Tang, Ming-De Jiang, Xiao-An Li
Wen Tang, Ming-De Jiang, Xiao-An Li, Department of Gastro-enterology, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China
Supported by the Military Medicine Scientific Research Foundation during 10th Five-Year Plan Period, No. 01MB037
Correspondence to: Dr. Ming-De Jiang, Department of Gastroenterology, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China. jiangmd88@yahoo.com.cn
Received: 2005-07-19 Accepted: 2005-08-05
Abstract
AIM: To explore the effect of sp600125, a special inhibitor of c-Jun terminal kinase (JNK), on the acetaldehyde-induced apoptosis of hepatic stellate cells-T6 (HSC-T6) and the expression of Caspase-3 protein in rats.
METHODS: The rat HSC-T6 induced by acetaldehyde was treated with different doses of sp600125. The proliferation of HSC-T6 was evaluated by MTT colorimetric assay, and the morphological changes of HSC-T6 were observed by Hoechst 33258 staining. The apoptotic rate of HSC-T6 was analyzed by flow cytometry (FCM), and the expression of Caspase-3 protein was examined by SABC method.
RESULTS: The proliferation of HSC-T6 was inhibited by different doses of sp600125 (F = 102.53, P <0.01). The apoptotic rate of HSC-T6 (F = 38.26, P <0.01) and the expression of Caspase-3 protein (F = 38.26, P <0.01) were significantly increased with the increasing of the sp600125 doses.
CONCLUSION: sp600125 can inhibit the proliferation and accelerate the apoptosis of HSC-T6, which may be related to the increased expression of Caspase-3 protein ......
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