杨竹林，邓星辉，杨乐平，李清龙，范文涛，梁 珊，苗雄鹰
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神经生长因子;二甲基苯并蒽;曲古霉素,杨竹林，邓星辉，杨乐平，李清龙，范文涛，梁珊，苗雄鹰，,杨竹林，,通讯作者：,收稿日期：,接受日期：,Expressionofnervegrowthfactorm
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杨竹林，邓星辉，杨乐平，李清龙，范文涛，梁珊，苗雄鹰，中南大学湘雅二医院肝胆胰疾病研究室湖南省长沙市 410011
杨竹林，男，1962-08-08生，湖南省涟源市人，汉族,1998年湖南医科大学博士，教授，博士生导师, 主要从事消化系统疾病的病理研究.
通讯作者：杨竹林, 410011, 湖南省长沙市, 中南大学湘雅二医院肝胆胰疾病研究室. zhulinyang@sina.com
收稿日期：2005-06-01 接受日期：2005-06-18
Expression of nerve growth factor messenger RNA in pancreatic cancer tissues in Sprague Dawley rats
Zhu-Lin Yang, Xing-Hui Deng, Le-Ping Yang, Qing-Long Li, Wen-Tao Fan, Shan Liang, Xiong-Ying Miao
Zhu-lin Yang, Xing-Hui Deng, Le-Ping Yang, Qing-Long Li, Wen-Tao Fan, Shan Liang, Xiong-Ying Miao, Laboratory of Hepatobiliary Diseases, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
Correspondence to: Zhu-Lin Yang, Laboratory of Hepatobiliary Diseases, the Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
Received: 2005-06-01 Accepted: 2005-06-18
Abstract
AIM: To establish a model of pancreatic cancer in Sprague-Dawley(SD)rats and to explore the expression and its significance of nerve growth factor(NGF)mRNA in pancreatic cancer and non-cancer tissues.
METHODS: Ninety rats were randomly divided into A (n = 40), B (n = 40) and C (n = 10) group. For group A and B, dimethylbenzanthrancene (DMBA) was directly implanted into the parenchyma of the rat pancreas to establish pancreatic cancer model. The rats in group B were treated with trichostatin(TSA). The rats in group C served as the controls. The rats were executed within 3-5 mo, and the carcinogenesis of the rats was observed by pathological methods. In situ hybridization was used to detect the expression of NGF mRNA in the formalin-fixed and routinely paraffin-embedded sections of pancreatic cancer and non-cancer tissues.
RESULTS: The prevalence rate of pancreatic cancer in group A was 48.7%(18/37) within 3-5 mo, 28.6%(2/7) at mo, 40.0%(4/10) at 4 mo and 60%(12/20) at 5 mo (P >0 ......
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