肝癌细胞BEL7402中神经元特异性烯醇化酶的表达
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朱爱萍，张青云， 王雅明， 徐建军，孙 丽
神经元特异性烯醇化酶;肝癌细胞;BEL7402细胞,朱爱萍，,张青云，,雅明，徐建军，孙丽，,通讯作者：,Expressionofhumanneur
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朱爱萍，山西医科大学第二医院检验科, 山西省太原市 030001
张青云，北京大学临床肿瘤学院 北京市肿瘤防治研究所免疫研究室 北京肿瘤医院检验科 北京市 100036
王雅明，徐建军，孙丽，北京大学临床肿瘤学院 北京市肿瘤防治研究所免疫研究室 北京市 100036
朱爱萍, 女, 1965-01-01生, 山西省太原市人, 汉族, 2004年山西医科大学免疫学硕士生, 副主任医师, 主要从事肿瘤相关酶类的研究.
北京市肿瘤分子生物学高技术实验室基金资助项目，No. 953850500
国家重点基础研究发展计划(973)，No. 2004CB518708分题
通讯作者：张青云, 100036, 北京市, 北京大学临床肿瘤学院, 北京肿瘤医院检验科, 北京市肿瘤防治研究所免疫研究室.qingyzhang@btamail.net.cn
电话：010-88115736
收稿日期：2005-07-28 接受日期：2005-08-03
Expression of human neuron-specific enolase gene in human hepatocellular cancer cells BEL7402
Ai-Ping Zhu, Qing-Yun Zhang, Ya-Ming Wang, Jian-Jun Xu, Li Sun
Ai-Ping Zhu, Department of Medical Laboratory, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Qing-Yun Zhang, Department of Medical Laboratory, Beijing Cancer Hospital, Peking University, Beijing 100036, China
Qing-Yun Zhang, Department of Immunology, Beijng Institute for Cancer Research, Beijing 100036, China
Qing-Yun Zhang, Ya-Ming Wang, Jian-Jun Xu, Li Sun, the School of Oncology, Peking University, Beijing 100036, China
Correspondence to: Dr. Qing-Yun Zhang, Department of Immunology, Beijng Institute for Cancer Research, Beijing 100036, China. qingyzhang@btamail.net.cn
Received: 2005-07-28 Accepted: 2005-08-03
Abstract
AIM: To investigate the expression of neuron-specific enolase(NSE) in hepatocellular cancer cells BEL7402 at the levels of transcription, protein and cells.
METHODS: The fragment of NSE gene was amplified from human hepatocellular cancer cell strain BEL7402 by reverse transcription polymerase chain reaction (RT-PCR) with NSE specific primers. The NSE expression was detected in the BEL7402 cells by Western blot and immunocytochemical (ICC) staining.
RESULTS: NSE product with a length of 1 305 bp was obtained by by RT-PCR. Western blot confirmed that Mr 50 000 NSE protein was expressed in BEL7402 cells ......
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