生存素 siRNA 表达质粒对MGC-803细胞增殖的影响
http://www.100md.com
赵伟红, 郭俊明, 肖丙秀, 管 忠,肖东升
生存素;RNA干扰;细胞周期;细胞增殖,生存素siRNA表达质粒对MGC-803细胞增殖的影响,赵伟红，郭俊明，肖丙秀，管忠，肖东升,，,赵伟红，,通讯作者：,EffectsofsurvivinsiR
参见附件(576kb)。
赵伟红，郭俊明，肖丙秀，管忠，肖东升，宁波大学医学院浙江省宁波市 315211
赵伟红，女，1966-07-12生，浙江省东阳市人，汉族，1989年浙江医科大学学士，2004年浙江大学医学院医学硕士，主要从事消化系统肿瘤研究.
浙江省医药卫生科学研究基金资助项目，No.2004A079
宁波市青年博士基金资助项目，No.2004A62000
通讯作者：郭俊明，315211，浙江省宁波市风华路818号,宁波大学医学院.junmingguo@yahoo.com
电话：0574-87600761 传真：0574-87608638
收稿日期：2005-07-28 接受日期：2005-08-06
Effects of survivin siRNA expression plasmid on proliferation of MGC-803 cells
Wei-Hong Zhao, Jun-Ming Guo, Bing-Xiu Xiao, Zhong Guan, Dong-Sheng Xiao
Wei-Hong Zhao, Jun-Ming Guo, Bing-Xiu Xiao, Zhong Guan, Dong-Sheng Xiao, Medical College of Ningbo University, Ningbo 315211, Zhejiang Province, China
Supported by Medical Science Research Fund of Zhejiang Province,No.2004A079; and Young Scientists Fund of Ningbo Municipality,No.2004A620007
Correspondence to: Jun-Ming Guo, Medical College of NingboUniversity, 818 Fenghua Road, Ningbo 315211, Zhejiang Province, China.junmingguo@yahoo.com
Received: 2005-07-28 Accepted:2005-08-06
Abstract
AIM: To silence the expression of survivin gene in MGC-803 cellsby the siRNA expression vector-based RNA interference (RNAi) technique,and to investigate its effects on the proliferation of MGC-803 cells.
METHODS: The survivin siRNA expression plasmid was transfectedinto MGC-803 cells by lipofectamine. Morphological changes of the cellswere observed under invert microscope. The expression of survivin mRNAwas determined by reverse transcription-polymerase chain reaction(RT-PCR). The changes of cell cycle and the cell proliferation wereanalyzed by flow cytometry and MTT assay, respectively.
RESULTS: Abnormal morphological changes of MGC-803 cells wereobserved in the group transfected with the survivin siRNA expressionplasmid. The survivin siRNA expression plasmid significantlydown-regulated the expression of survivin mRNA in MGC-803 cells with apercentage of 48.2% ( vs empty controls), and it arrested thecell cycle in G1 phase (77.4%). The cell proliferation was significantlyinhibited, and the optical density in siRNA-transfected cells wasmarkedly lower than that in the empty controls (24 h: 0.272 ± 0.048 vs0.576 ± 0.018; 48 h: 0.270 ± 0.060 vs 0.809 ± 0.027; 72 h:0.143 ± 0.046 vs 1.015 ± 0.075; all P <0.01). Thegrowth inhibitory rates of MGC-803 cells were 53.4%, 66.7%, and 86.3%after 24, 48, and 72 h of the transfection, respectively.
CONCLUSION: The expression of survivin in MGC-803 cells can bedown-regulated by the plasmid-based RNAi technique, and thedown-regulation can inhibit the cell proliferation in vitro.
Key Words: Survivin; RNA interference; Cell cycle; Cellproliferation
Zhao WH, Guo JM, Xiao BX, Guan Z, Xiao DS. Effects of survivin siRNAexpression plasmid on proliferation of MGC-803 cells. Shijie Huaren Xiaohua Zazhi2005;13(19):2302-2305
摘要
目的：应用siRNA表达载体介导的RNAi技术 ......
赵伟红，郭俊明，肖丙秀，管忠，肖东升，宁波大学医学院浙江省宁波市 315211
赵伟红，女，1966-07-12生，浙江省东阳市人，汉族，1989年浙江医科大学学士，2004年浙江大学医学院医学硕士，主要从事消化系统肿瘤研究.
浙江省医药卫生科学研究基金资助项目，No.2004A079
宁波市青年博士基金资助项目，No.2004A62000
通讯作者：郭俊明，315211，浙江省宁波市风华路818号,宁波大学医学院.junmingguo@yahoo.com
电话：0574-87600761 传真：0574-87608638
收稿日期：2005-07-28 接受日期：2005-08-06
Effects of survivin siRNA expression plasmid on proliferation of MGC-803 cells
Wei-Hong Zhao, Jun-Ming Guo, Bing-Xiu Xiao, Zhong Guan, Dong-Sheng Xiao
Wei-Hong Zhao, Jun-Ming Guo, Bing-Xiu Xiao, Zhong Guan, Dong-Sheng Xiao, Medical College of Ningbo University, Ningbo 315211, Zhejiang Province, China
Supported by Medical Science Research Fund of Zhejiang Province,No.2004A079; and Young Scientists Fund of Ningbo Municipality,No.2004A620007
Correspondence to: Jun-Ming Guo, Medical College of NingboUniversity, 818 Fenghua Road, Ningbo 315211, Zhejiang Province, China.junmingguo@yahoo.com
Received: 2005-07-28 Accepted:2005-08-06
Abstract
AIM: To silence the expression of survivin gene in MGC-803 cellsby the siRNA expression vector-based RNA interference (RNAi) technique,and to investigate its effects on the proliferation of MGC-803 cells.
METHODS: The survivin siRNA expression plasmid was transfectedinto MGC-803 cells by lipofectamine. Morphological changes of the cellswere observed under invert microscope. The expression of survivin mRNAwas determined by reverse transcription-polymerase chain reaction(RT-PCR). The changes of cell cycle and the cell proliferation wereanalyzed by flow cytometry and MTT assay, respectively.
RESULTS: Abnormal morphological changes of MGC-803 cells wereobserved in the group transfected with the survivin siRNA expressionplasmid. The survivin siRNA expression plasmid significantlydown-regulated the expression of survivin mRNA in MGC-803 cells with apercentage of 48.2% ( vs empty controls), and it arrested thecell cycle in G1 phase (77.4%). The cell proliferation was significantlyinhibited, and the optical density in siRNA-transfected cells wasmarkedly lower than that in the empty controls (24 h: 0.272 ± 0.048 vs0.576 ± 0.018; 48 h: 0.270 ± 0.060 vs 0.809 ± 0.027; 72 h:0.143 ± 0.046 vs 1.015 ± 0.075; all P <0.01). Thegrowth inhibitory rates of MGC-803 cells were 53.4%, 66.7%, and 86.3%after 24, 48, and 72 h of the transfection, respectively.
CONCLUSION: The expression of survivin in MGC-803 cells can bedown-regulated by the plasmid-based RNAi technique, and thedown-regulation can inhibit the cell proliferation in vitro.
Key Words: Survivin; RNA interference; Cell cycle; Cellproliferation
Zhao WH, Guo JM, Xiao BX, Guan Z, Xiao DS. Effects of survivin siRNAexpression plasmid on proliferation of MGC-803 cells. Shijie Huaren Xiaohua Zazhi2005;13(19):2302-2305
摘要
目的：应用siRNA表达载体介导的RNAi技术 ......
您现在查看是摘要介绍页，详见PDF附件(576kb)。