摘要：目的：探讨虎参痛风胶囊(Hus)对高尿酸血症小鼠T淋巴细胞亚群的影响。方法：小鼠按随机化分组原则分为空白组、别嘌呤醇组、模型组及5%，2.5%，1.25%虎参痛风胶囊组，灌胃给药6 d后，采用腹腔注射黄嘌呤一次造模，30 min后摘眼球取血，尿酸酶比色法测定血尿酸；处死小鼠，取脾脏，剪碎后，制备成单细胞悬液，单色法应用流式细胞仪测定CD3+，CD4+，CD8+百分比及CD4+/CD8+比值。结果：模型组血尿酸明显升高(与空白组比较，P<0.05)，而别嘌呤醇组和5%，2.5%，1.25%虎参痛风胶囊组能明显降低血尿酸(与模型组比较，P<0.05)。应用流式细胞仪检测脾脏T淋巴细胞分型结果显示：模型组、别嘌呤醇组和空白组CD3+，CD4+，CD8+及CD4+/CD8+均无显著性差异；且5%虎参痛风胶囊组与模型组、空白组、别嘌呤醇组相比较亦无显著性差异。结论：Hus具有明显的降低高尿酸血症小鼠血尿酸的作用，优于别嘌呤醇。急性高尿酸血症和Hus对脾脏T淋巴细胞亚群无明显影响。

    关键词：虎参痛风胶囊；高尿酸血症；T淋巴细胞亚群

    Influence in the Subpopulations of T Lymphocytes Administrated Hus in Mice

    CHEN Chao

    (Dept. of Pharmacology of Three Gorges University, Yichang, Hubei, 443003, China)

    Abstract:Objective:To elucidate the influence in the subpopulations of T lymphocyte administrated Hus in mice.Methods:Adopt 10% xanthine i.p. in mice to duplicate hyperuricemia model,and administrate drugs for 6 days beforehand. Then, to detect serum urate concentration by uricase chromatometry, and to measure the percentage of CD3+、CD4+、CD8+and CD4+/CD8+ ratio of spleen T lymphocytes by monochromic flow cytometry (FCM).Results:5%,2.5%,1.25% Hus group and allopurinol group had significant differentiation respectively compared with model group (P<0.01 ......