【摘要】目的 观察锂-匹鲁卡品(LPC)致痫大鼠杏仁核神经元损伤的形态学改变，并检测细胞凋亡相关的DNA碎片标志物。方法用LPC诱发大鼠癫痫持续状态(status epilepticus，SE)3 h，于癫痫中止后第24小时和第48小时处死动物。一组鼠用于制作脑切片，分别用光镜和电镜观察；另一组从相同的脑区提取DNA，在含有溴乙锭的琼脂糖凝胶上电泳。注射了生理盐水的大鼠被用作阴性对照。结果在第24小时和第48小时两个时点，SE 大鼠杏仁核神经元出现了形态学上的坏死和梯状DNA电泳带，对照组未见到坏死及凋亡细胞。结论癫痫持续状态后，形态学上表现为坏死的神经元出现梯状DNA电泳带，提示在此模型中可能存在程序性细胞死亡的机制。

    【关键词】癫痫持续状态；凋亡；坏死；DNA梯状电泳带

    Mechanism of neuronal death in nucleus amygdalae of rats after lithium-pilocarpine induced status epilepticus

    LIANG JueyinSUN Jianying FENG Yanqiu et al

    Department of Neurology，Shandong Provincial Hospital, Jinan, 250021

    【Abstract 】Objective To observe the morphology of neuronal death in nucleus amygdalae of rats after status epilepticus(SE) induced by lithium-pilocarpine(LPC), and correlate this with markers of DNA fragmentation that has been associated with cellular apoptosis. MethodsSE was induced for 3 hours with LPC.24 or 48 hours later the rats were killed.One group had brain sections stained， then examined by light and electron microscopy . DNA was extracted from the same area and electrophoresed on an agarose gel with ethidium bromide . The same rats injected with saline were used as negative controls .Results24 and 48 hours after SE ......