摘要：目的：运用PAdEasy系统构建了带荧光蛋白的人CTLA-4Ig腺病毒载体，为进一步研究CTLA-4Ig诱导免疫耐受的机制奠定了基础。方法：采用基因技术，将目的基因克隆至穿梭质粒pAdTrack-CMV中，利用PAdEasy系统进行重组，然后在293细胞中进行扩增。并进行病毒滴度测定。体外感染人肾小管上皮细胞。结果：酶切鉴定和PCR证明重组人CTLA-4Ig腺病毒载体构建正确。结论：应用PAdEasy细菌内重组技术成功构建了人CTLA-4Ig带荧光腺病毒载体。

    关键词：腺病毒;CTLA-4Ig;同源重组

    The Express and Recombinant of Adenovirus of Human CTLA-4Ig Gene

    FENG Ning-han, ZHANG Wei, et al

    (The First Affiliated Hospital to Nanjing Medical College,Jiangsu Nanjing 210029, China)

    Abstract: Objective: To construct the recombinant adenovirus of human CTLA-4Ig gene .Method: The CTLA-4Ig gene was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination.Result: It is confirmed that the CTLA-4Ig gene were inserted successfully into the adenovirus by using restriction endonuclease and PCR analyses .Conclusion: The recombinant adenovirus of human CTLA-4Ig gene was successfully constructed.

    Key words:Adenovirus；CTLA-4；Homogenic reconstruction

    器官移植是治疗许多终末期疾病的唯一有效方法 ......