RP-HPLC法测定拉米夫定棕榈酸酯脂质体药物含量及包封率
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胡会国，金圣煊，王艳芝，邓意辉，毕殿洲
(沈阳药科大学 药学院，辽宁 沈阳 110016)
摘要：目的 建立拉米夫定前体药物拉米夫定棕榈酸酯脂质体含量及包封率的测定方法。方法 反相高效液相色谱法(RP-HPLC)，采用DiamonsilTM ODS C18 (4.6 mm×200 mm, 5 mm) 色谱柱，流动相为甲醇-水(体积比为95∶5)，流速为1 mL·min-1，紫外检测波长为270 nm。采用超速离心法分离拉米夫定棕榈酸酯脂质体中的游离药物。结果 在本色谱条件下辅料和试剂对药物测定无干扰，拉米夫定棕榈酸酯在3.42×10-6～8.55×10-5 mol·L-1 内线性关系良好(r＝0.999 9)，平均回收率为100.7%(n＝9)，精密度的日内RSD及日间RSD均小于2%(n＝5)。结论 该方法准确可靠、简单快速，可用于拉米夫定棕榈酸酯脂质体含量及包封率的测定。
关键词: 药剂学；包封率；反相高效液相色谱法；拉米夫定棕榈酸酯；药物脂质体
中图分类号：R94 文献标识码：A
RP-HPLC determination of the content and entrapment efficiency of lamivudinyl palmitate liposomes
HU Hui-guo, JIN Sheng-xuan, WANG Yan-zhi, DENG Yi-hui, BI Dian-zhou
(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)
Abstrat: Objective To establish a RP-HPLC method for content and entrapment efficiency determination of lamivudinyl palmitate liposomes. Method The separation was performed with a DiamonsilTM ODS C18 (4.6 mm×200 mm, 5 mm) using methanol-water (V∶V=95∶5) as the mobile phase. The flow rate was 1 mL·min-1. It was detected at 270 nm. Result The calibration curve was linear within the range of 3.42×10-6～8.55×10-5 mol·L-1 (r=0.999 9), the intra-day RSD and inter-day RSD were less than 2%, and the average recovery was l00.7% (n＝9). Conclusion This method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of lamivudinyl palmitate liposomes.
Key words: pharmaceutics; entrapment efficieny; RP-HPLC; lamivudinyl palmitate; drug liposome
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