CyclinD1和P16在宫颈上皮内瘤变及宫颈鳞癌中的表达及其意义
CyclinD,,宫颈鳞癌;,宫颈上皮内瘤样变(CIN);,P16;,细胞周期蛋白CyclinD1;,免疫组织化学,1资料与方法,2结果,3讨论,参考文献:
摘要: 目的: 探讨CyclinD1、P16在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)、宫颈鳞癌(squamous cell carcinoma of cervix uteri, SCC)组织中的表达及意义。方法: 应用免疫组化技术 (SP法)检测CyclinD1蛋白和P16蛋白在54例宫颈鳞癌组织(SCC组)、20例宫颈上皮内瘤样病变(CINⅢ组)的表达， 20例慢性宫颈炎组织作对照组，结合临床病理特征进行分析。结果:(1)在对照组CyclinD1蛋白几乎未见表达，在CIN 组和SCC组中表达强度增高，与对照组比较，差异均有统计学意义(P<0.05)。CIN 组和SCC组组间比较差异无统计学意义(P>0.05)。(2)SCC组中随着临床分期增加， CyclinD1蛋白的表达强度逐渐增高，差异有统计学意义(P<0.05)。(3)P16蛋白在对照组、CIN 组和SCC组中表达强度逐渐降低，但是3组比较差异无统计学意义(P>0.05)。结论：(1) CyclinD1蛋白可能在宫颈癌的发生发展中起一定的作用。(2) CyclinD1蛋白在SCC组中的表达与临床分期有关,其过度表达提示宫颈鳞癌预后不良。(3) P16的失活可能参与部分宫颈鳞癌的发生。关键词: 宫颈鳞癌; 宫颈上皮内瘤样变(CIN); P16; 细胞周期蛋白CyclinD1; 免疫组织化学
Expression and significance of cyclinD1, P16 in cervical carcinoma and cervical intraepithelial neoplasia
ZHOU Yongmei, LI Tinfang
(Department of Obstetrics and Gynecology, Xinjiang BaZhou People′s Hospital, Kuoerle 841000, China)
Abstract: Objective： To study the expression and significance of cyclinD1, P16 proteins in cervical carcinoma and cervical intraepithelial neoplasia. Methods： The protein of cyclinD1 and P16 in 20 normal cervical tissue, 20 cervical intraepithelial neoplasia, 54 cervical carcinoma were tested with immunohistochemical technique (straptavidin biotin peroxidase SP). Results：(1) There was significant difference in the express of cyclinD1 proteins between normal cervical tissue and cervical carcinoma(P<0.05). There was significant difference in the express of cyclinD1 proteins between normal cervical tissue and cervical intraepithelial neoplasia (P<0.05). No Significant difference was found in the express of cyclinD1 proteins between cervical intraepithelial neoplasia and cervical carcinoma (P>0.05). (2) CyclinD1 protein was associated with clinical stage (P<0.05). (3) There was not significant difference in the express of P16 proteins between three groups. Conclusion： (1) cyclinD1 proteins expression may play an important role in the cervical carcinoma and cervical intraepithelial neoplasia. (2) Expression of cyclinD1 protein in cervical carcinoma was associated with clinical stage. (3) Inactivation of P16 may play role in the initiation of cervical carcinoma partly. ......
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