MsPGN_____系膜增生性肾小球肾炎NFκBp65;

MsPGN中活化NFκBp65mRNA、IL1βmRNA、IL4mRNA的检测及其意义

http://www.100md.com 《新疆医科大学学报》 2005年第8期

MsPGN,,系膜增生性肾小球肾炎NFκBp65;,细胞因子;,系膜细胞;,原位杂交,1材料和方法,2结果,3讨论,参考文献:

     摘要: 目的：研究系膜增生性肾小球肾炎(MsPGN)肾组织中活化核因子κBp65、IL1β及IL4基因表达的意义，探讨MsPGN的发病机制。方法：应用原位杂交技术分别检测正常对照组9例与病例组41例MsPGN肾组织中NFκBp65mRNA、IL1βmRNA及IL4 mRNA水平，并分析3项检测指标之间的相互关系及其与MsPGN病变程度的关系。结果：(1)正常对照组中肾小球系膜区活化NFκBp65mRNA、IL1DmRNA阳性检出率分别为11.1%、33.3%，而IL4 mRNA为0；病例组3项检测指标阳性率分别为70.7%、75.6%、61.1%。2组肾小球中3项检测指标阳性率差异有统计学意义(P<0.05)。(2) MsPGN肾小球组织中NFκBp65mRNA活化与IL1βmRNA之间呈显著正相关(r=0.383 6，P<0.05)，IL1βmRNA与IL4mRNA之间亦呈显著正相关(r=0.477 1，P<0.01)。(3)活化NFκBp65mRNA、IL1βmRNA及IL4mRNA阳性率均随着病变程度加重增高，但差异无统计学意义。(4)正常肾组织及MsPGN的肾小管中3项指标检测结果无一定规律性。结论：3项指标在MsPGN发病环节中共同起着作用。MsPGN对致病原的反应更接近于Th2反应。

    关键词：系膜增生性肾小球肾炎NFκBp65; 细胞因子; 系膜细胞; 原位杂交

    Detection of activated NFκBp65mRNA, ILlβmRNA and IL4mRNA inmesangial proliferative glomerulonephritis and its

    siginificanceLIU Xia, ZHANG Wei, WEN Hao

    (Department of Pathology, First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054, China)Abstract: Objective: To study the roles of activated nuclear factorkaapB (NFκB) p65mRNA, Interleukin1β(IL1β) mRNA and Interleukin4 (IL4) mRNA in the pathogenesis of mesangial proliferative glomerulonephritis, and discuss the potential the raptatic way in renal inflammatory. Methods: To use in situ hybridization (ISH) to detect activated nuclear factorkaapB (NFκB) p65mRNA, Interleukin1β (IL1) mRNA, Interleukin4 (IL4) mRNA in biopsy specimens of kidney from patients with mesangial proliferation glomerulonephritis (MsPGN) (n=41) and in structurall normal kidneys (n=9). Results: (1) Weak staining of Activated NF κBp65 , IL1β in glomerulus of normal kidney(11.1%,33.3%), inter leukin4 positive cells were not found in the normal control kidney tissues, the positive rates of threephase detecting index are higher in glomerulus of MsPGN than in normal kidney(P<0.05); (2)The positive rate of activated NF κBp65mRNA is showed a positive correlation with IL1β mRNA (r=0.383 6, P<0.05) and also positive correlation with IL1βmRNA and IL4mRNA(r=0.477 1, P<0.05). (3)A correlation was found between the positive rate of actived NFκBp65mRNA, I11β mRNA, IL4mRNA with the degree of Mesangial proliferation, but with no statistic significance. (4) There are not certain regularity in the tubular cells of normal kidney and MsPGN. Conclusion: Activated NFκBp65mRNA , IL1 13 mRNA and Ⅱ4mRNA are associated with the development of MsPGN. The reactive of Th2 may have relation with the pathogenesis of MsPGN ......

百拇医药网 http://www.100md.com/html/DirDu/2005/11/05/79/23/78.htm

您现在查看是摘要页，全文长 10534 字符。