增殖细胞核抗原_

结直肠癌组织中CyclinB1、p34cdc2、PCNA和Ki67表达的研究

http://www.100md.com 《中国普通现代外科进展》 2005年第5期

增殖细胞核抗原,,结直肠肿瘤·增殖细胞核抗原·CyclinB1·p34cdc2·Ki67,1材料方法,2结果,3讨论,参考文献

     【摘要】目的：探讨结直肠癌发生和分化中CyclinB1、p34cdc2、PCNA和Ki67表达异常及其临床病理学意义。方法：采用免疫组化SABC法检测CyclinB1、p34cdc2、PCNA和Ki67在16例结直肠癌组织中蛋白的表达。结果：CyclinB1和p34cdc2染色定位于细胞胞浆和(或)胞核内。PCNA和Ki67染色定位于胞核内。在研究病例中有87.5%(14/16)CyclinB1阳性表达，81.2%(13/16)p34cdc2阳性表达，93.7%(15/16)PCNA阳性表达，81.2%(13/16)Ki67阳性表达。低分化结直肠癌的CyclinB1、p34cdc2、PCNA和Ki67阳性率明显高于高中分化结直肠癌，CyclinB1、p34cdc2、PCNA和Ki67的表达具有高度一致性。结论：CyclinB1、p34cdc2、PCNA和Ki67的阳性表达率及表达强度同结直肠癌分化程度有密切关系。

    【关键词】结直肠肿瘤·增殖细胞核抗原·CyclinB1·p34cdc2·Ki67

    Expression of CyclinB1,p34cdc2,PCNA and Ki67 in colorectal carcinogenesis

    BIAN Yuehong,MA Xiaoli,,HUANG Shuhong,HUANG Jingai,ZHAI Yonghua,XIE Jingwu,ZHANG Hongwei

    1Institute of Development Biology,School of Life Science,Shandong University (Jinan 250100,China)

    2Department of Surgery,Qilu Hospital of Shangdong University (Jinan 250012,China)

    3Center Laboratory of Jinan Central Hospital,Clinical Medical College of Shandong University (Jinan 250013,China)

    4Department of Phamacology and Toxicology,University of Texas Medical Branch

    (Galveston 775551048 USA)

    【ABSTRACT】Objective:To research the clinicopathological significance of CyclinB1,p34cdc2,PCNA and Ki67 in colorectal carcinogenesis.Methods:The expression of CyclinB1,p34cdc2,PCNA and Ki67 was examined by SABC immunohistochemical technique in colorectal carcinogenesis.Results:Immunoreaction to CyclinB1 and p34cdc2 was mainly localized in the cytoplasm or nuclear of cells.Expression of PCNA and Ki67 was localized in the nucleus. The postive immunostaining rate of CyclinB1,p34cdc2,PCNA and Ki67 was 87.5%,81.2%,93.7% and 81.2%,respectively.CyclinB1,p34cdc2,PCNA and Ki67 positive rates in poorly differentiated colorectal carcinoma were much higher than those in well differentiated colorectal carcinoma. Conclusion:CyclinB1,p34cdc2,PCNA and Ki67 play a certain role in the development of colorectal carcinoma,and as malignant degree of colorectal carcinoma increases,their positive rates and grades will be higher. ......

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