干扰素γ__

γ干扰素对HBV相关性肝癌细胞凋亡的免疫调节效应研究

http://www.100md.com 《中国普通现代外科进展》 2005年第5期

干扰素γ,,干扰素γ，重组·肝肿瘤·肝炎病毒，乙型·配体·细胞凋亡·免疫，细胞,1材料与方法,2结果,3讨论,参考文献

     【摘要】目的：以转染HBV全基因组并稳定分泌病毒抗原的HepG2.2.15肝癌细胞系为细胞模型,探索IFNγ对HBV相关性肝癌的免疫调节效应。方法：用HBsAg 和HBeAg ELISA检测试剂盒测定IFNγ作用0，6，12，24h后，HBV病毒颗粒的分泌表达水平。通过MTT法检测不同剂量IFNγ对新型凋亡配体TRAIL与TWEAK的生长抑制效应的调节作用,不同剂量IFNγ对TRAIL与TWEAK的凋亡诱导效应的调节作用。结果：IFNγ在不同的时间点对HBsAg和HBeAg的分泌均具有抑制作用，IFNγ能够显著增强TRAIL和TWEAK对HepG2.2.15肝癌细胞系的生长抑制效应和凋亡率。讨论：IFNγ是HBV感染后的一个重要的免疫效应分子，它可以通过抑制病毒复制和增强免疫监视分子的效应而在HBV相关性肝癌中发挥抗病毒和抗肿瘤效应。

    【关键词】干扰素γ，重组·肝肿瘤·肝炎病毒，乙型·配体·细胞凋亡·免疫，细胞

    Immune regulation effect of IFNγ on HBV related hepatocarcinoma

    HAN Lihui,SUN Wensheng,JIA Xiaoqing,MA Chunhong,GAO Lifen,LIU Suxia,WANG Xiaoyan

    Immunology Institute of Medical School,Shandong University (Jinan 250012,China)

    【ABSTRACT】Objective:The HBV transgenic HepG2.2.15 hepatocarcinoma cell line was used as a cell model to explore the immune regulation effect of IFNγ on the HBV related hepatocarcinoma.Methods:The HBV particles secreted by HepG2.2.15 cells were detected by HBsAg and HBeAg detecting ELISA kits.Modulation of IFNγ to the the growth inhibiton effect induced by apoptosis inducing ligand TRAIL and TWEAK were assayed by MTT method.Modulation of IFNγ to the apoptosis inducing effect of TRAIL and TWEAK were assayed by flow cytometry.Results: Secretion of HBsAg and HBeAg could be inhibited by IFNγ.The growth inhibition effect and apoptosis induced by TRAIL and TWEAK could be greatly increased by IFNγ.Conclusion:IFNγ is a very important immune effective molecule that will play a key role after HBV infection.It could exert its immune function by inhibiting the virus secretion and enhance the immune surveillance in HBV related hepatocarcinoma. ......

百拇医药网 http://www.100md.com/html/DirDu/2005/11/05/79/25/97.htm

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