摘要：目的：研究急性呼吸窘迫综合征(ARDS)大鼠肾脏Gq/11蛋白含量的动态变化及其意义。方法: 32只雄性Wistar大鼠尾静脉注射油酸(oleic acid, OA)复制ARDS模型(油酸组),免疫印迹法测定肾脏Gq/11蛋白含量。结果: 与对照组相比，随时间延长OA组PaO2、MAP逐渐降低(P<0.05或0.01)， BUN、Cr升高(P<0.01)，血浆和肾脏LDH活性及MDA浓度增高(P<0.01)，血浆ACE活性较对照组降低(P<0.01)，而肾脏ACE活性则随时间延长逐渐增高 (P<0.01)。OA组肾脏Gq/11蛋白含量高于对照组(P<0.05或0.01)。结论: Gq/11蛋白含量增加在ARDS并发肾脏损伤中有一定作用。

    关键词：G蛋白；信号转导；急性呼吸窘迫综合征；肾脏；大鼠

    The change of Gq/11 protein content in rat kidney during ARDS

    ZHANG Li, Kelala, Parhat, et al

    (Department of Physiology, Xinjiang Medical University, Urumqi 830054, China)

    Abstract: Objective: To investigate the dynamic changes of Gq/11 protein in kidney during acute respiratory distress syndrome(ARDS). Methods: To establish ARDS model in rats by intravenous injection of oleic acid (OA 0.2 ml/Kg-1) though tail vein. The content of Gq/11 were assessed by Western blot. Results: MAP and PaO2 of OA groups decreased (P<0.05 or 0.01), but BUN and Cr content, LDH activity and MDA content of plasma and kidney significantly increased (P<0.01). ACE activity of plasma gradually reduced (P<0.01). On the contrary, ACE activity of kidney increased. The content of Gq/11 obviously increased (P<0.05 or 0.01). Conclusions: Up regulation of Gq/11 protein content may play a role in renal injury during ARDS. ......