乙醇诱导大鼠急性胃炎后Fos在中枢神经系统的表达
Fos蛋白,,急性胃炎;Fos蛋白;中枢神经系统,1材料和方法,2结果,3讨论,参考文献:
摘要: 目的: 探讨乙醇诱导大鼠急性胃炎后Fos在中枢神经系统的表达及其意义。方法: 取30只成年雄性Wistar大鼠,随机分为5组,每组6只。A组:单纯生理盐水灌胃组;B组:单纯乙醇灌胃组;C组:膈下迷走神经切断乙醇灌胃组;D组:内脏大、小神经切断乙醇灌胃组;E组:膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。运用免疫组化技术显示Fos阳性神经元在大鼠中枢神经系统(CNS)中的分布。 结果: B、C组动物的Fos阳性神经元在丘脑腹后外侧核、第9胸髓后角胶状质内大量存在,疑核和迷走神经背核内有少量聚集;B、D组Fos阳性神经元在疑核和迷走神经背核内有较多聚集,但远低于B、C组在丘脑腹后外侧核、第9胸髓后角胶状质内的Fos阳性神经元数。而A、E组在上述4个核团内Fos阳性神经元则较少。结论: 胃的痛觉冲动以交感神经传入为主。关键词: 急性胃炎;Fos蛋白;中枢神经系统
Expression of Fos in central nevrous system after ethanolinduced acute gastritis in rat
GAN Ziming, LIU Jun, CHEN Shengguo, et al
(Department of Human Anatomy, Xinjiang Medical University, Urumqi 830054, China)
Abstract: Objective: To study the expression and significance of cfos in central nervous system after ethanolinduced acute gastritis in rat. Methods: Animals were randomly classified into 5 groups: group A, gasterpoured by physiological solution; group B, gasterpoured by ethanol; group C, gasterpoured by ethanol after hypophrenic vagectomy; group D, gasterpoured by ethanol after bigsmall splanchnicotomy; group E, gasterpoured by ethanol after hypophrenic vagotomy and bigsmall splanchnicotomy.The distribution of cfos positive neuron in central nervous system was displayed and immunohistochemical staining method was used. Results: There were a lot of cfospositive neuron in thalamus ventrallateral nucleus and No 9 thoracic medullaris posterior horn cinerea in groups B and C; Fos positive neuron in ambiguous nucleus and dorsal nucleus of vagus nerve were better in groups B and D, but they were obiviously less than them of halamus ventrallateral nucleus and No 9 thoracic medullaris posterior horncinerea in groups B and C. Conclusion: The gastric algesia impulsion are mainly conducted by sympathetic afferent nerve. ......
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