胃癌患者p53基因突变的检测及其临床意义
胃癌,,胃癌；p53基因；聚合酶链反应单链构像多态性分析法；银染,1材料与方法,2结果,3讨论,参考文献:
摘要: 目的：探讨胃癌患者癌组织及外周血p53基因突变表达情况，评价p53基因作为胃癌患者术后预后判定、转移监测及指导临床治疗的应用价值。方法：利用聚合酶链反应单链构像多态性分析法(PCRSSCP)和银染技术检测32例胃癌患者外周血、癌旁正常组织、癌组织中p53基因外显子5、6、7、8位点及20例健康人外周血p53基因的突变情况，并比较不同组织类型、不同分期、有无淋巴结转移胃癌患者p53基因的突变率。结果：32例胃癌患者癌旁正常组织和20例正常人外周血中未检出p53基因突变；胃癌患者癌组织中的p53基因突变率为37.5%(12/32)，与癌旁正常组织无相比差异有统计学意义(P<0.05)；有淋巴结转移患者的胃癌组织p53基因突变高于无淋巴结转移患者(P<0.05)；胃癌患者外周血p53基因突变率为15.6%(5/32)，外周血中p53基因突变阳性者其相应癌组织p53基因突变均为阳性，经过3个月随访，其中有1例确诊为肝转移。不同组织类型不同分期胃癌患者p53基因突变率差异均无统计学意义(P>0.05)。结论：p53基因突变对胃癌患者术后预后判定、转移监测及指导临床治疗有一定的意义。关键词: 胃癌；p53基因；聚合酶链反应单链构像多态性分析法；银染
Measure and significance of mutation of p53 gene in human gastric cancer
ZHAO Zeliang, WANG Haijiang, YIN Dong, et al
(Department of Abdmominal Surgery, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830000, China)
Abstract: Objective: To analyse the p53 gene mutation on exon 5, 6, 7, 8, compare the rate of mutation of p53 gene between circulation blood and tissue of gastric cancer, estimate its value on diagnosis, metastases and therapy in gastric cancer. Method: PCRSSCP and silver dying were used to circulating blood and tissue of gastric cancer. Rusults: Mutation of p53 gene was identified in 12 of 32 cases in tissue the rate of 37.5%, and 5 of 32 in blood with rate of 15.6% respectively, all of mutated cases in blood also mutated in its corresponding tissue, and correlate to lymph node metastases. Conclusion: The circulating p53 mutation reflects the p53 mutation in tissue of gastric cancer, and it can be used as a new method of gene diagnoses and a useful metastasis factor. ......
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