Nm23H1基因对膀胱癌化疗敏感性的体外实验研究
H1基因,,膀胱癌;,nm23H1基因;,化疗敏感性,1材料与方法,2结果,3讨论,参考文献:
摘要 ： 目的: 探讨外源性人抑癌基因nm23H1对膀胱癌细胞系T24细胞化疗敏感性的影响。方法： 将nm23H1真核表达质粒通过脂质体转染技术导入人膀胱癌细胞株T24，利用免疫组化及Westernblot法鉴定质粒的表达。利用MTT法、流式细胞仪分析技术检测T24细胞转染前后化疗药物对细胞增殖、细胞凋亡的变化。结果： 顺铂组(CDDP)细胞生长明显受到抑制，细胞凋亡增加；顺铂转染前后细胞凋亡指数分别为(26.51±0.98)%、(63.21±0.58)%，两者间差异有统计学意义。结论： Nm23H1基因能增强顺铂的化疗敏感性,为基因联合化疗药物治疗膀胱癌提供了参考依据。关键词: 膀胱癌; nm23H1基因; 化疗敏感性
An experimental study of effect of nm23H1 gene on the chemotherapeutic sensitivity of bladder cancer in vitro
CUI Shu, HUANG Jin xing, YANG Jiankuen, et al
(Department of Urology, Secend Affiliated Hospital, Xinjiang Medical University,Urumqi 8300038, China)
Abstract: Objective: To evaluate the effect of exogenous human tumor suppressor gene nm23H1 on the chemotherapeutic sensitivity of the bladder cancer cell lines T24 cell. Methods: the plasmid of nm23H1 was transferred into human bladder cancer cell lines T24 cell by the method of lipofect transfermation and the expression of nm23H1 were assayed by means of immunohistochemical technique and Western blot MTT assay was used to assess the cell proliferation effect of the chemotherapeutic medicines on the bladder cancer cell lines T24 cell transferred by nm23H1, and the cell apoptosis was measured by flow cytometry. Result: The grows of T24 cell with cisplatin(CDDP), transferred by nm23H1 ......
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