血清早孕因子_____孕妇;_血清早孕因子;

孕妇血清中早孕因子的分离与纯化

http://www.100md.com 《新疆医科大学学报》 2005年第4期

血清早孕因子,,孕妇;,血清早孕因子;,分离纯化;,分子量,1材料与方法,2结果,3讨论,参考文献:

     摘要：目的：建立一条从妊娠早期妇女血清中分离纯化早孕因子的技术路线，获得高纯度的早孕因子。方法：采用依次经过DEAE 52阴离子交换层析、SP Sepharose F.F阳离子交换层析、ConA Sepharose 4B亲和层析和Heparin Sepharose Cl6B亲和层析分离纯化的方案，最终得到具有早孕因子活性的HeparinⅡ成分，早孕因子活性采用活性玫瑰花环抑制实验检测。采用SDS聚丙烯酰胺凝胶电泳鉴定纯化物。结果：SDS聚丙烯酰胺凝胶电泳结果显示有一条电泳区带，相对分子量为10.91 kDa。结论：这条分离纯化早孕因子的技术路线是可行的，得到的相对分子量为10.91 kDa的蛋白质为电泳纯的早孕因子。

    关键词：孕妇; 血清早孕因子; 分离纯化; 分子量

    Isolation and purification of Early Pregnancy Factor from Human Pregnancy Sera

    LIU Xiaoyu, SUN Wendong, YUAN Wumei, et al

    (Department of Biochemistry and Molecular Biology, Xinjiang Medical University,Urumqi 830054,China)

    Abstract: Objective: Set up a method of isolation and purification of early pregnancy factor(EPF) from human pregnancy sera, and get pure EPF. Methods: The purification scheme involved sequential DEAE 52 chromatography, SP Sepharose F.F chromatography, ConA Sepharose 4B chromatography and Heparin Sepharose Cl6B chromatography. The biological activity was detected by rosette inhibition test. It was Identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE). Result: One band was observed on SDSPAGE profile, and the molecular weight was 10.91 kDa. Conclusion: This method is advisable. The purified protein has a molecular mass of 10.91 kDa as analyzed by SDSPAGE, which is similar to the report of Cavanagh AC. ......

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