慢性牙周炎患者龈下菌斑中齿垢密螺旋体的PCR检测
牙周炎,,慢性牙周炎；齿垢密螺旋体；,多聚酶链反应,1材料与方法,3讨论,参考文献:
摘要: 目的：探讨龈下菌斑中齿垢密螺旋体(Td)的检出率与慢性牙周炎临床指标的关系。方法：取58例慢性牙周炎患者患病部位及相对健康部位龈下菌斑,用终浓度为1%的Triton X100 及100℃ 水浴10 min处理标本从而制备DNA 模版，对齿垢密螺旋体16s rRNA 保守区片段进行扩增。结果：58例 患者中47例(81.0%)患病部位PCR为阳性，8例(13.8%)相对健康部位PCR为阳性。结论：(1) 齿垢密螺旋体在慢性牙周炎患者龈下菌斑中患病部位检出率高于相对健康部位，是慢性牙周炎致病菌之一,且随Td 检出率的增高，慢性牙周炎的严重程度有逐渐加重趋势。(2) PCR 检测Td敏感性强、特异性高。关键词: 慢性牙周炎；齿垢密螺旋体； 多聚酶链反应
Detection of treponema denticola from subgingival plaque of patients withchronic periodontitis by PCR
Gulibositan，Adalati ，LIN Ren yong, et al
(Department of Stomatology , First Affliated Hospital ,Xinjiang Medical University, Urumqi 830054, China)
Abstract: Objective: To detect the relationship between the presence of treponema denticola(Td) and chronic periodontitis. Methods: Subgingival plaque samples were taken from disease sites and healthy sites of 58 patients with chronic periodontitis and treated with 1%Triton X100 and 100℃ for 10 minutes to prepare DNA templates. The fragments of 16s rRNA gene detected by PCR. Result: 47 of 58 (81.0%) subgingival samples from disease sites and 8 of 58(13.8%) healthy sites showed positive PCR results. Conclusion: (1)The detection rate of Td in disease sites was much higher than the healthy sites, it must be the putative etiologic agent in chronic periodontitis and Td infection was close related to the severity of chronic periodontitis.(2) PCR was sensitive and distinctive method to detect Td. ......
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