庆祝中国首台原子吸收光谱仪诞生40周年暨主要研制者吴廷照教授从事化学分析事业55年、原子吸收光谱事业发展研讨会征文
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庆祝中国首台原子吸收光谱仪诞生40周年 暨主要研制者吴廷照教授从事化学分析事业55年、原子吸收光谱事业发展研讨会征文.PDF
Study on Determ i na tion of Polyphenols i n Honeysuckle Flower By
M i crocolumn Hi gh Performance L iquid Chroma tography
Hu Qiufen
1, 2
, Yang Guangyu
2
, Huang Zhangjie
2
, L i Haitao
1
, Yin J iayuan
3 2
1
(Depart m ent of Chem istry, Yux i Teacher′ s College, Yuxi 653100)
2
(Depart m ent of Chem istry, Yunnan Univer sity, Kunm ing 650091)
Abstract A microcolumn high perfor mance liquid chromat ography (HPLC) method for the determinati on of
polyphenols ( chl orogenic acid, quercitrin2 32 glucoside, luteolin2 72 glucoside, luteolin2 72 galact oside,quercitrin, luteolin) in honeysuckle fl ower was studied . The polyphenols were extracted from the samp le by
methanol with refluxing for 45 min . Then the polyphenolswas separated on aWaters Xterra
T M
RP18 (1 . 0 mm ×
50 mm, 2 . 5μm) micr ocolumn with methanol and 1% acetic acid s oluti on gradient eluti on asmobile phase at ......
M i crocolumn Hi gh Performance L iquid Chroma tography
Hu Qiufen
1, 2
, Yang Guangyu
2
, Huang Zhangjie
2
, L i Haitao
1
, Yin J iayuan
3 2
1
(Depart m ent of Chem istry, Yux i Teacher′ s College, Yuxi 653100)
2
(Depart m ent of Chem istry, Yunnan Univer sity, Kunm ing 650091)
Abstract A microcolumn high perfor mance liquid chromat ography (HPLC) method for the determinati on of
polyphenols ( chl orogenic acid, quercitrin2 32 glucoside, luteolin2 72 glucoside, luteolin2 72 galact oside,quercitrin, luteolin) in honeysuckle fl ower was studied . The polyphenols were extracted from the samp le by
methanol with refluxing for 45 min . Then the polyphenolswas separated on aWaters Xterra
T M
RP18 (1 . 0 mm ×
50 mm, 2 . 5μm) micr ocolumn with methanol and 1% acetic acid s oluti on gradient eluti on asmobile phase at ......