[摘要] 目的：应用随意引物聚合酶链反应(arbitrarily primed polymerase chain reaction, AP-PCR)来对健康青少年口腔优势菌进行鉴定。方法：经初步鉴定健康青少年口腔优势菌为血链球菌属后，用随机引物AP-PCR对优势菌进行扩增、检测。结果：经扩增后的健康青少年口腔优势菌的DNA片段与标准血链球菌ATCC10556的DNA片段相同。结论：应用AP-PCR技术可以对健康青少年口腔优势菌进行鉴定和分类。

    [关键词] 随意引物聚合酶链反应 鉴定 健康青少年 口腔优势菌

    Arbitrarily Primed Polymerase Chain reaction for Genotypic Identification of Predominant Bacteria In Oral Cavity of Healthy Adolescent.

    WU Shuang-yan, SUN De-gang, MA Sheng-li.

    Qing Dao Stomatological Hospital, Qing Dao 266003

    [Abstract]Objective: To identify predominant bacteria in oral cavity of healthy adolescent by arbitrarily primed polymerase chain reaction (AP-PCR). Methods: After predominant bacteria in oral cavity of healthy adolescent was identified primarily to Streptococcus .sanguis group , AP-PCR was used by designing arbitrary primer 5′AAG AGA GGA GCT AGC TCT TCT TGG A3. Results: The main DNA ′band of predominant bacteria in oral cavity of healthy adolescent is similar to Streptococcus .sanguis ATCC10556 ′. Conclusion: AP-PCR can be useful in identification of predominant bacteria in oral cavity of healthy adolescent. ......