【摘要】 目的 研究调心方有效部位(TX0201)对Aβ25-35所致类AD模型大鼠脑组织神经生长因子(NGF)及其受体p75调节机制的异同。方法 采用双侧杏仁核注射Aβ25-35造成类AD大鼠模型，观察大鼠Morris水迷宫空间记忆能力，并分别通过免疫组化和RT-PCR方法研究类AD大鼠神经元NGF及其受体p75和APP mRNA变化以及TX0201的影响。结果 类AD模型大鼠Morris水迷宫测试出现空间记忆能力下降，APP mRNA表达提高，神经生长因子减少，神经系统失去了营养保护。TX0201能有效控制以上病理变化。结论 TX0201提高模型大鼠定向学习记忆能力，可能通过降低皮层、海马部位APP mRNA的表达，增加NGF阳性样细胞、减少p75受体阳性样细胞调节神经营养而发挥作用。提示通过有效调节神经生长因子是TX0201的重要作用机制之一。

    【关键词】 老年性痴呆；调心方有效部位；多奈哌齐；β-淀粉样蛋白；神经生长因子

    Effects of TX0201 on nerve growth factor and p75 in rats

    YANG Ge，LIN Shui-miao，ZHAO Wei-kang，et al.

    Xiyuan Hospital，Academy of Traditional Chinese Medicine，Beijing 100091，China

    【Abstract】 Objective To research the mechanism of TX0201 abstracted from‘Heart-Regulating Formula’in treatment of rat with Alzheimer’s disease(AD).Methods The rat model of AD were induced by Aβ25-35 injected into the bilateral amygdala.To observe the spatial learning and memory ability，and by means of RT-PCR and immunohistochemistry analysis methods，in the brain of the model animal，the expression of β-amyloid precursor protein(APP)，nerve growth factor(NGF)and its receptor were examined.Results In AD model group ......