【摘要】 目的 通过2种检测登革病毒方法的比较，以提高检测登革病毒的灵敏度和特异性。方法 利用Taqman MGB技术，根据登革病毒3’端非编码区的一段高度保守序列，设计登革1～4型荧光PCR通用引物和TaqmanMGB探针，以登革热毒株作为标准，以乙脑毒株作对照，建立实时荧光PCR检测登革病毒的快速方法。并对10份ELISA法检测阳性的临床血清标本进行RT-PCR及荧光PCR扩增。结果 RT-PCR检测10份临床血清标本，2份阳性，阳性率为20%。实时荧光PCR检测登革病毒与乙脑病毒无交叉反应，检测10份临床血清标本，5份阳性，阳性率为50%。从RNA提取到检测结果仅需4h。结论 Taqman MGB实时PCR检测方法快速、敏感性高、特异性强，可作为登革病毒的快速检测方法，应用于登革热的临床早期诊断。

    【关键词】 登革病毒；RT-PCR；Taqman GMB实时PCR

    Comparative study of detecting dengue viruses with real-time fluorescent PCR and RT-PCR

    LIU Jian-jun， GU Li-bahaer， YANG Fan， et al.

    Shenzhen Center for Diseases Control and Prevention，Shenzhen 518020，China

    【Abstract】 Objective In order to improve the sensitivity and specificity of detecting dengue viruses， two methods for detecting dengue viruses were compared.Methods Using Taqman MGB technique， a pair of universal primers and Taqman MGB probe were designed according to a highly reserved sequence of the 3’-noncoding region of dengue viruses type 1-4. Dengue virus strains were used as standard and Japanese encephalitis virus strains were used as control， the real-time PCR assay for specific and sensitive detection of the dengue viruses was established. While 10 serum specimens of ELISA positive were detected by the RT-PCR and fluorescent PCR.Results There was no cross-reaction with Japanese encephalitis virus. Of 10 specimens ......