冷冻保存后成人胰岛细胞与小肠黏膜下层的共培养
http://www.100md.com
侯 军, 薛武军, 冯新顺, 田小辉, 庞新路,
冷冻保存;回收率;胰岛功能;小肠黏膜下层侯军,薛武军,冯新顺,田小辉,庞新路,藤焱.冷冻保存后成人胰岛细胞与小肠黏膜下层的共培养.世界华人消化杂志2005;13(22)2697-26990引言,冷冻保
 |
| 第1页 |
参见附件(852KB，3页)。
侯军, 薛武军, 冯新顺, 田小辉, 庞新路,藤焱, 西安交通大学医学院第一附属医院肾移植科陕西省西安市 710061 通讯作者:薛武军, 710061,陕西省西安市健康路1号,西安交通大学医学院第一附属医院肾移植科. xuewujundoctor@sina.com
电话: 029-85324033 传真: 029-85324033
收稿日期: 2005-08-29 接受日期: 2005-09-27
Co-culture of post-cryopreservedhuman pancreatic islet cells with small intestinal submucosa
Jun Hou, Wu-Jun Xue, Xin-Shun Feng, Xiao-Hui Tian, Xin-Lu Pang, Yan Teng
Jun Hou, Wu-Jun Xue,Xin-Shun Feng, Xiao-Hui Tian, Xin-Lu Pang, Yan Teng, Department ofRenal Transplantation, the First Affiliated Hospital of Xi’an JiaotongUniversity, Xi’an 710061, Shaanxi Province, China
Correspondence to: Wu-Jun Xue, Department of RenalTrans-plantation, the First Affiliated Hospital of Xi’an JiaotongUniversity, 1 Jiankang Road, Xi’an 710061, Shaanxi Province,China.xuewujundoctor@sina.com
Received: 2005-08-29 Accepted:2005-09-27
Abstract
AIM:To investigate the supportive and protective effect of thesmall intestinal submucosa (SIS) on the post-cryopreserved humanpancreatic islet cells.
METHODS:The purified human islet cells were divided into SISand control group after they were cryopreserved for 1 mo. The recoveryrate of the islet cells was determined after they were cultured inRPMI-1640 for 1 wk. The morphological features and insulin excretion afterstimulation were assessed in vitro.
RESULTS:The recovery rate of the islet cells in SIS group wassignificantly higher than that in the control group (90.5 ± 1.8% vs62.7 ± 3.6%, P < 0.05), and the cells were well shaped. Afterstimulation, the insulin secretion in the SIS group was markedly higherthan that in the control group (25.8 ± 1.7 mU/L vs 14.6 ± 1.3mU/L, P < 0.05). When the islet cells were placed in the highglucose solution containing theophylline, the calculated stimulation indexin SIS group was about 3 times as high as that in the control group.
CONCLUSION:The co-culture of post-cryopreserved humanpancreatic islet cells with small intestinal submucosa is a promising wayto increase recovery rate and improve the function of thepost-cryopreserved islets ......
您现在查看是摘要介绍页，详见PDF附件(852KB，3页)。