核糖体蛋白L5在胃癌中的表达及功能
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刘 骥, 李纪鹏, 陈冬利, 张洪伟, 王为忠
核糖体蛋白;胃癌;siRNA,刘骥,李纪鹏,陈冬利,张洪伟,王为忠,刘骥,通讯作者:,Expressionandfunctionalstudyofribosom
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刘骥, 李纪鹏, 陈冬利, 张洪伟, 王为忠, 中国人民解放军第四军医大学西京医院胃肠外科 陕西省西安市 710032
刘骥, 男, 1978-06-23生, 江苏省泗阳人, 汉族, 第四军医大学2003级普通外科学硕士, 医师, 主要从事胃癌的基础研究工作.
通讯作者: 王为忠, 710032, 陕西省西安市, 中国人民解放军第四军医大学西京医院胃肠外科. weichang@fmmu.edu.cn
电话: 029-83375265
收稿日期: 2005-09-28 接受日期: 2005-10-19
Expression and functional study of ribosomal protein L5 in gastric cancer
Ji Liu, Ji-Peng Li, Dong-Li Chen, Hong-Wei Zhang, Wei-Zhong Wang
Ji Liu, Ji-Peng Li, Dong-Li Chen, Hong-Wei Zhang, Wei-Zhong Wang, Department of Gastrointestinal Surgery, Xijing Hospital of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Correspondence to: Wei-Zhong Wang, Department of Gastrointestinal Surgery, Xijing Hospital of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. weichang@fmmu.edu.cn
Received: 2005-09-28 Accepted: 2005-10-19
Abstract
AIM: To investigate the expression of ribosomal protein L15 (RPL5) in gastric cancer as well as its effect on the proliferation of gastric cancer cells.
METHODS: The expression of RPL5 was detected in gastric cancer cell lines AGS, MKN45, SGC7901 and MGC803 by Western blot. The specific siRNA vector of RPL5 was constructed and then transfected into AGS cells. The expression of RPL5 in the transfectants was examined by Western blot. The growth of transfected cells was evaluated by MTT assay and flow cytometry.
RESULTS: The expression of RPL5 in gastric cancer cell lines AGS, MKN45, SGC7901 and MGC803 were all significantly higher than that in GES-1 and normal epithelial cells of gastric mucosa. The specific siRNA vectors of RPL5, named U6-RPL5A and U6-RPL5B, were successfully constructed and transfected into AGS cells. U6-RPL5A could inhibit the expression of RPL5 significantly. The growth rate of U6-RPL5A transfected cells, named AGS-U6-RPL5A, was lower than the control cells, and the percentage of the cells that was in proliferation phase was decreased by about 5% ......
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