实验性肝纤维化大鼠肝脏中 TIMP-2 基因及蛋白表达阻断研究
http://www.100md.com
聂青和, 张亚飞, 谢玉梅, 邵 彬, 李 军,
反义寡核苷酸;金属蛋白酶组织抑制因子-2;肝纤维化;大鼠,聂青和,张亚飞,谢玉梅,邵彬,李军,苟艳子,周永兴
 |
| 第5页 |
 |
| 第1页 |
参见附件(1137KB，6页)。
聂青和, 张亚飞, 谢玉梅, 邵彬, 李军, 苟艳子, 周永兴, 第四军医大学唐都医院传染病科/全军感染病诊疗中心 陕西省西安市 710038
聂青和, 男, 汉族, 1961年生, 医学博士、博士后, 主要从事传染病学临床与实验研究, 以第一作者发表论文及综述150余篇，主编学术专著3部, 现任《中国实用内科杂志》、《世界华人消化杂志》、《胃肠病学和肝病学杂志》、《肝脏》、《中国感染控制杂志》、《寄生虫病与感染性疾病》等8家学术专业杂志编委,《实用肝脏病杂志》副主编,《中华传染病杂志》、《中华消化杂志》特邀审稿人.
陕西省科技攻关项目(社发攻关), No.2003K10G63
通讯作者: 聂青和, 710038, 陕西省西安市新寺路1号, 中国人民解放军第四军医大学唐都医院传染病科、全军感染病诊疗中心. nieqinghe@hotmail.com
电话: 029-84777852 传真: 029-83537377
收稿日期: 2005-10-25 接受日期: 2005-11-04
Antisense oligonucleotides-based inhibition of gene and protein expression of tissue inhibitor of metalloproteinase-2 on experimental liver fibrosis in rats
Qing-He Nie, Ya-Fei Zhang, Yu-Mei Xie, Bin Shao, Jun Li, Yan-Zi Gou, Yong-Xing Zhou
Qing-He Nie, Ya-Fei Zhang, Yu-Mei Xie, Bin Shao, Jun Li, Yan-Zi Gou, Yong-Xing Zhou, Chinese PLA Centre of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
Supported by the Key Science and Technology Project of Shaanxi Province, No. 2003K10G63
Correspondence to: Dr. Qing-He Nie, Chinese PLA Centre of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China. nieqinghe@hotmail.com
Received: 2005-10-25 Accepted: 2005-11-04
Abstract
AIM: To investigate the inhibitory effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) targeted antisense oligonucleotide (asON) on the progress of experimental liver fibrosis in rats.
METHODS: Twenty-two rats were divided into asON treatment (n = 6), model (n = 6), and normal control group (n = 10). The liver fibrosis model was induced by human serum albumin (HSA). During modeling, the rats were treated with antisense oligonucleotides via tail vein. The level of TIMP-2 expression was detected by reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization, immunohistochemical staining and enzyme linked immunosorbent assay (ELISA) ......
您现在查看是摘要介绍页，详见PDF附件(1137KB，6页)。