Ss-A/Ro 核蛋白 60 ku 亚单位及其变异体在耐药胃癌细胞中的表达
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韩全利, 丁 杰, 郭长存, 王 新, 乔泰东,
胃癌;Ss-A,Ro60 ku亚单位;变异体;多药耐药,Ss-A,Ro核蛋白60ku亚单位及其变异体在耐药胃癌细胞中的表达,韩全利,丁杰,郭长存,王新,乔泰东,张学庸,樊代明,通讯作者
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韩全利, 空军总医院干部病房三区 北京市 100036
丁杰, 郭长存, 王新, 乔泰东, 张学庸, 樊代明, 第四军医大学西京医院全军消化病研究所 陕西省西安市 710032
通讯作者: 韩全利, 100036, 北京市海淀区阜成路30号, 空军总医院干部病房三区. hanquanli@21cn.com
电话: 010-66928132 传真: 010-66928132
收稿日期: 2005-09-10 接受日期: 2005-10-10
Expression of Ss-A/Ro ribonucleoprotein 60 ku subunit and its variants in drug-resistant gastric cancer cells
Quan-Li Han, Jie Ding, Chang-Cun Guo, Xin Wang, Tai-Dong Qiao, Xue-Yong Zhang, Dai-Ming Fan
Quan-Li Han, the Third Section of Gerontology Department, General Hospital of Air Force of Chinese PLA, Beijing 100036, China
Jie Ding, Chang-Cun Guo, Xin Wang, Tai-Dong Qiao, Xue-Yong Zhang, Dai-Ming Fan, Institute of Digestive Diseases, Xijing Hospital of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Correspondence to: Dr. Quan-Li Han, the Third Section of Gerontology Department, General Hospital of Air Force of Chinese PLA, 30 Fucheng Road, Haidian District, Beijing 100036, China. hanquanli@21cn.com
Received: 2005-09-10 Accepted: 2005-10-10
Abstract
AIM: To clone the encoding gene of Ss-A/Ro ribonucle-oprotein 60 ku subunit, and to detect the expression of Ss-A/Ro 60 ku subunit and its variants in the drug resi-stance gastric cancer cells.
METHODS: The encoding gene of Ss-A/Ro ribonucl-eoprotein 60 ku subunit was cloned by reverse transcri-ption-polymerase chain reaction. The expression of Ss-A/Ro 60 ku subunit and its variants in the gastric cancer cells were analyzed by semi-quantitative RT-PCR.
RESULTS: The Ss-A/Ro 60 ku subunit was successful-ly cloned, and its two transcription variants, with a length of 52 and 41 bp, respectively, were found out ......
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