应激对大鼠结肠神经系统 nNOS 表达的影响
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李玉梅, 陆国明
应激;肠神经系统;nNOS;免疫组化,应激对大鼠结肠神经系统nNOS表达的影响,李玉梅,陆国明,通讯作者:,Effectofstressonexpressionofneuronalnitricox
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李玉梅, 武警浙江省总队医院消化科 浙江省嘉兴市 314000
陆国明, 嘉兴学院医学院组织胚胎学教研室 浙江省嘉兴市 314001
李玉梅, 女, 1966-07-30生, 浙江省嘉兴市人, 汉族, 2002年第三军医大学硕士, 副主任医师, 主要从事消化系疾病的临床及基础研究.
浙江省嘉兴市科技发展基金资助项目, No.20041041
通讯作者: 李玉梅, 314000, 浙江省嘉兴市南湖路16号, 武警浙江省总队医院消化科. li.yumei@sohu.com
电话: 0573-2852851-34537 传真: 0573-2852933
收稿日期: 2005-10-25 接受日期: 2005-11-12
Effect of stress on expression of neuronal nitric oxide synthase in colonic nervous system in rats
Yu-Mei Li, Guo-Ming Lu
Yu-Mei Li, Department of Gastroenterology, Zhejiang Crops Hospital, Chinese People’s Armed Police Forces, Jiaxing 314000, Zhejiang Province, China
Guo-Ming Lu, Department of Histology and Embryology, Medical College of Jiaxing University, Jiaxing 314001, Zhejiang Province, China
Supported by the Jiaxing Science and Technology Development Foundation of Zhejiang Province, No. 20041041
Correspondence to: Dr. Yu-Mei Li, Department of Gastroenterology, Zhejiang Crops Hospital, Chinese People’s Armed Police Forces, 16 Nanhu Road, Jiaxing 314000, Zhejiang Province, China. li.yumei@sohu.com
Received: 2005-10-25 Accepted: 2005-11-12
Abstract
AIM: To investigate the effect of stress on the expression of neuronal nitric oxide synthase (nNOS) in colonic nerv-ous system in rats.
METHODS: Thirty male SD rats were randomly divided into control group, stress group and NG-nitro-L-arginine methyl ester (L-NAME) group. The rat model of water immersion-restraint stress (WRS) was established. The expression of nNOS in colonic submucous plexus and myenteric plexus in the rats was examined by immuno-histochemical staining and analyzed by computer image analysis system.
RESULTS: nNOS immune-positive substance was mostly expressed in the neurons of submucous plexus and myenteric plexus. In comparison with that in contr-ol group, the gray value of nNOS positive neurons in submucous plexus and myenteric plexus was signifi-cantly decreased (P = 0 ......
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