吴茱萸_____吴茱萸；氨基酸；含量测定；HPLC

吴茱萸中氨基酸的HPLC分析

http://www.100md.com 《河北北方学院学报(医学版)》 2005年第3期

吴茱萸,,吴茱萸；氨基酸；含量测定；HPLC,1仪器与试剂,2实验部分,3方法学考察,4样品分析,参考文献

     【摘要】目的：用HPLC法测定吴茱萸中氨基酸的含量。方法：以水为溶剂用超声震荡提取法提取游离氨基酸。提取液用HPLC法分析，色谱柱：YWG-C18(10μm,250mm×46mm)，流动相：水-甲醇-乙腈(42∶20∶38)，流速：10ml/min，柱温：30℃，检测波长：260nm。结果：实验中发现吴茱萸中含有多种游离氨基酸，而且不同批次吴茱萸中游离氨基酸的种类和含量不同。结论：本文建立了用HPLC法测定吴茱萸中氨基酸的分析方法并对其中13种氨基酸进行了定性定量分析。

    【关键词】吴茱萸；氨基酸；含量测定；HPLC

    Analysis of Amino Acid in Evodia Rutaecarpa(Juss.)Benth by HPLC

    LU Yi1,LIU Shufen1,ZHEN Pan2

    1.Environmentl Protection Bureau of Changli, County, Changli， 066000,China;

    2.Hebei North University,Zhangjiakou,075000,China

    【ABSTRACT】 Objective:To establish a method for determing amino acid in Evodia rutaecarpa(Juss.)Benth by HPLC. Methods:After the amino acid was extracted with water as solvent,the extrated fluid was performed on YWG-C18( 46mm×250mm, 10μm)column with the mobile phase of watermethanolacetonitrile(42∶20∶38). The flow rate was 10mL/min.The wavelength of UV detector was 260 nm and the column temperature was 30℃. Results: There were many kinds of amino acids in Evodia rutaecarpa(Juss.)Benth .The contents and types of amino acid in different samples were different. Conclusion: The method for determing amino acid by HPLC was establish and 13 types of amino acid were analysised in Evodia rutaecarpa(Juss.)Benth. ......

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