荧光定量PCR检测CK20在直肠癌外周血中表达的意义
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殷红专, 韩 霞, 苏 琪, 吴硕东, 刘恩卿
荧光定量PCR;直肠癌;CK20,殷红专,韩霞,苏琪,吴硕东,刘恩卿,通讯作者:,电话:,收稿日期;,接受日期;,DetectionofCK20
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殷红专, 韩霞, 苏琪, 吴硕东, 刘恩卿, 中国医科大学附属盛京医院普外科 辽宁省沈阳市110004
通讯作者: 殷红专, 110004, 辽宁省沈阳市和平区三好街36号, 中国医科大学盛京附属医院普外科. chad156@sina.com
电话: 024-82154257
收稿日期; 2005-09-21 接受日期; 2005-09-30
Detection of CK20 in peripheral blood of rectal cancer patients with fluorescence quantitative polymerase chain reaction and its significance
Hong-Zhuan Yin, Xia Han, Qi Su, Shuo-Dong Wu, En-Qing Liu
Hong-Zhuan Yin, Xia Han, Qi Su, Shuo-Dong Wu, En-Qing Liu, Department of General Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Hong-Zhuan Yin, Department of General Surgery, the Affiliated Shengjing Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China. chad156@sina.com
Received: 2005-09-21 Accepted: 2005-09-30
Abstract
AIM: To analyze the relations of CK20 expression in the peripheral blood with the Dukes stages and histological types, and to discuss the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) for predicting the metastases and prognosis of rectal cancer.
METHODS: The peripheral blood samples were collected from 47 patients with rectal cancer, 10 patients with benign intestinal diseases, and 10 healthy volunteers. Then FQ-PCR was performed to detect the expression of CK20 mRNA in the peripheral blood.
RESULTS: There was no positive expression of CK20 mRNA in the patients with benign intestinal diseases and the healthy volunteers, which was significantly different from that in the patients with rectal cancer (72.34%, 34/47) (χ2 = 37.434, P <0.01). The level of CK20 expression in the peripheral blood of the 47 patients with rectal cancer was positively correlated with the Dukes stages of rectal cancer (r = 0.680, P <0.01).
CONCLUSION: The level of CK20 expression is positively related with tumor stages in the peripheral blood of the rectal cancer patients ......
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