ALA-PDT 诱导SW480细胞凋亡和胞内 Ca2+ 浓度变化的关系
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郑江华, 时 德, 陈祖林
δ氨基酮戊酸;光动力疗法;SW480细胞;细胞内游离钙;凋亡,ALA-PDT诱导SW480细胞凋亡和胞内Ca2+浓度变化的关系,郑江华,时德,陈祖林,通讯作者:,电话:,传真:,收稿日期:,接受日
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郑江华, 时德, 重庆医科大学附属第一医院血管外科 重庆市 400016
陈祖林, 第三军医大学新桥医院普外科 重庆市 400037
郑江华, 男, 1975-12-23生, 四川省广安市人, 汉族, 博士研究生, 主要从事血管外科及肿瘤综合治疗的临床与基础研究.
国家自然科学基金资助项目, No. 30271481
通讯作者: 陈祖林, 400037, 重庆市, 重庆市第三军医大学新桥医院普外科. chenzulin@163.com
电话: 023-68774405 传真: 023-68774605
收稿日期: 2005-09-20 接受日期: 2005-10-19
Relationship between intracellular Ca2+ and ALA-PDT induced apoptosis in SW480 cells
Jiang-Hua Zheng, De Shi, Zu-Lin Chen
Jiang-Hua Zheng, De Shi, Department of Vascular Surgery, the First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China
Zu-Lin Chen, Department of General Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China
Supported by National Natural Science Foundation of China, No. 30271481
Correspondence to: Dr. Zu-Lin Chen, Department of General Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China. chenzulin@163.com
Received: 2005-09-20 Accepted: 2005-10-19
Abstract
AIM: To investigate relationship between intracellular Ca2+ and apoptosis induced by aminolaevulinic acid-photodynamic therapy (ALA-PDT) in SW480 cells.
METHODS: SW480 cells were divided into control, light, ALA and ALA-PDT group. The corresponding treatment was performed in each group. The apoptosis of SW480 cells was detected by DNA fragment assay and TUNEL assay. The changes of intracelluar Ca2+ concentration in each group were observed by confocal laser scanning microscopy.
RESULTS: DNA ladder formation of apoptotic features was demonstrated 1 and 2 h after ALA-PDT treatment. The apoptosis index (AI) of 30 and 60 min after ALA-PDT treatment was 25.26 ± 5.04% and 50.45 ± 7.85%, respectively, which were significantly higher than those in the other 3 groups (all AI <10%, P <0.01). The concentration of intracelluar Ca2+ 20 min after ALA-PDT treatment was markedly increased than that at 10 min (fluorescent intensity: 185 ......
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