丙型肝炎病毒干扰素敏感决定区聚合酶链反应检测方法的建立及应用
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张剑平, 徐道振, 闫 杰, 何忠平, 董庆鸣,
丙型肝炎病毒;干扰素敏感区;聚合酶链反应,张剑平,徐道振,闫杰,董庆鸣,戴旺苏,沈冰,魏红山,成军,何忠平,通讯作者:,电话:,传真:
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张剑平, 徐道振, 闫杰, 董庆鸣, 戴旺苏, 沈冰, 魏红山, 成军, 北京地坛医院传染病研究所 北京市 100011
何忠平, 北京佑安医院检验科 北京市 100054
通讯作者: 张剑平, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. dtyyzhjp@163.com
电话: 010-64211031-2358 传真: 010-64281540
收稿日期: 2005-09-28 接受日期: 2005-10-11
Establishment and application of polymerase chain reaction method for detection of interferon sensitivity determining region of hepatitis C virus
Jian-Ping Zhang, Dao-Zhen Xu, Jie Yan, Zhong-Ping He, Qing-Ming Dong, Wang-Su Dai, Bing Shen, Hong-Shan Wei, Jun Cheng
Jian-Ping Zhang, Dao-Zhen Xu, Jie Yan, Qing-Ming Dong, Wang-Su Dai, Bing Shen, Hong-Shan Wei, Jun Cheng, Institute of Infectious Diseases, Beijing Ditan Hospital, Beijing 100011, China
Zhong-Ping He, Department of Clinic Laboratory, Beijing Youan Hospital, Beijing 100054, China
Correspondence to: Dr. Jian-Ping Zhang, 100011, Institute of Infectious Diseases, Beijing Ditan Hospital, 13 Ditan Park of Anwai Street, Dongcheng District, Beijing, China. dtyyzhjp@163.com
Received: 2005-09-28 Accepted: 2005-10-11
Abstract
AIM: To establish a polymerase chain reaction (PCR) method based on the special primers of the interferon sensitivity determining region (ISDR) of hepatitis C virus (HCV), and to detect the sequence of the HCV ISDR.
METHODS: The universal outer primers and 3 suits of ISDR type-specific inner primers (A, B, C group) were designed on the basis of the nucleotide sequences of 6 typical full-genomic HCV. The HCV RNA in the serum that colleted from 27 patients infected with chronic HCV genotype 1b was tested by the established PCR method, and multi-site analysis was performed on the PCR positive products.
RESULTS: The positive rats were 37% (10/27), 30% (8/27), 4% (1/27), and 22% (6/27) in group A, group A-B, group A-C, and group A-B-C after detection by the established PCR method ......
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