发酵莲子乳对小鼠胃肠道运动、吸收功能的调节作用
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吴小南, 陈 洁, 汪家梨, 林建银
幽门螺杆菌;细胞凋亡;胃上皮;细胞;P53;bax;bcl-2;表达,姜海行,聂海明,邓德海,覃山羽,陶霖,黄振宁,通讯作者:,电话:,传真:,收稿日
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姜海行, 聂海明, 邓德海, 覃山羽, 陶霖, 黄振宁, 广西医科大学第一附属医院 广西壮族自治区南宁市 530021
姜海行, 男, 1963-08-22生, 广西省柳州市人, 汉族, 1986年广州中山医科大学医学系毕业, 2001年获博士学位, 教授, 主要从事幽门螺杆菌及胃癌发病机制研究.
通讯作者: 姜海行, 530021, 广西壮族自治区南宁市双拥路22号, 广西医科大学第一附属医院消化内科. jihaxi@263.net
电话: 0771-5356501 传真: 0771-5353668
收稿日期: 2005-10-25 接受日期: 2005-10-31
Helicobacter pylori induces apoptosis of rat gastric epithelial cells in vitro
Hai-Xing Jiang, Hai-Ming Nie, De-Hai Deng, Shan-Yu Qin, Lin Tao, Zhen-Ning Huang
Hai-Xing Jiang, Hai-Ming Nie, De-Hai Deng, Shan-Yu Qin, Lin Tao, Zhen-Ning Huang, Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China
Correspondence to: Dr. Hai-Xing Jiang, Department of Gastro-enterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China. jihaxi@263.net
Received: 2005-10-25 Accepted: 2005-10-31
Abstract
AIM: To investigate the effect of Helicobacter pylori sonicated extract on the apoptosis of rat gastric epithelial cells as well as the expression of apoptosis-related genes in vitro.
METHODS: H pylori sonicated extract from strain Sydney SS-1 was cultured with OUMS-37, a kind of immortalized rat gastric cell lines. Apoptosis of the cells was confirmed according to specific changes of morphology and DNA ladder 24-48 h after co-incubation. The expression of P53 protein was detected by Western blotting and the expression of bax and bcl-2 mRNA were observed by Northern blotting.
RESULTS: The specific morphology of the cells such as shrinkage, condensation, margination of nuclear chromatin and apoptotic bodies were observed under light microscope. DNA ladder was manifested by fragment analysis. Western blotting showed a dose-dependent increased expression of wild-type P53 protein and Northern blotting showed a dose-pendent increased expression of bax mRNA and reduced expression of bcl-2 mRNA in the treated cells ......
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