【摘要】 目的 本实验研究脓毒血症大鼠肾组织匀浆TNF-α、MCP-1含量变化。方法 通过对SD大鼠盲肠结扎及贯穿，建立大鼠的脓毒血症模型，分别取肾组织通过组织裂解液，形成组织匀浆液，取匀浆上清测定TNF-α、MCP-1含量。结果 在SD大鼠脓毒症早期，肾组织匀浆中TNF-α、MCP-1含量明显增高。结论 TNF-α、MCP-1是脓毒血症早期激活的细胞因子，脓毒症引起肾功能改变与细胞因子大量释放有关。

    【关键词】 脓毒症；肿瘤坏死因子-α；单核细胞趋化蛋白-1

    Effects of sepsis early on SD rats renal expression of TNF-α、MCP-1

    REN Yan-bo，ZHANG Yu，JIANG Li.

    Department of Emergency ICU，Dalian Medical Collage Hospital，Dalian 116011，China

    【Abstract】 Objective To observe the changs of SD rats renal levels of tumor necrosis factor-α(TNF-α)、monocyte chemotactic protein-(MCP-1)in sepsis early.Methods Sepsis was produced in rats by local ligation and puncture.Changes in TNF-α、MCP-1 levels in renal tissues were determined by enzyme-linked immunosorbent assay ......