肝门阻断对大鼠肠道肌间神经丛内 NOS 阳性神经元的影响
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张军明, 任艳华, 刘 芬, 雷亚宁, 张 婵,
肝门阻断;肠肌间神经丛;一氧化氮合酶;肠神经系统;大鼠,肝门阻断对大鼠肠道肌间神经丛内NOS阳性神经元的影响,张军明,任艳华,刘芬,雷亚宁,张婵,钱旭,通讯作者:,Effectsofportaltr
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张军明, 任艳华, 刘芬, 雷亚宁, 张婵, 钱旭, 温州医学院组织胚胎学教研室 浙江省温州市 325035
温州医学院科技发展基金资助项目, No. XNK04038
通讯作者: 张军明, 325035, 浙江省温州市, 温州医学院组织胚胎学教研室. wmczjm@wzmc.net
电话: 0577-86689967
收稿日期: 2005-11-15 接受日期: 2005-12-02
Effects of portal triad clamping on nitric oxide synthase positive neurons in myenteric plexus of rat intestinal tract
Jun-Ming Zhang, Yan-Hua Ren, Fen Liu, Ya-Ning Lei, Chan Zhang, Xu Qian
Jun Li, Ya-Juan Zhao, Mei Sun, Department of Pediatrics, the Second Affiliated Hospital of China Medical University. Shenyang 110004, Liaoning Province, China
Jun-Ming Zhang, Yan-Hua Ren, Fen Liu, Ya-Ning Lei, Chan Zhang, Xu Qian, Department of Histology and Embryology, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China
Supported by the Technical Development Fund of Wenzhou Medical College, No. XNK04038
Correspondence to: Jun-Ming Zhang, Department of Histology and Embryology, Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China. wmczjm@wzmc.net
Received: 2005-11-15 Accepted: 2005-12-02
Abstract
AIM: To explore the nervous mechanism of intestinal function disorder after portal triad clamping through observing the changes of nitric oxide synthase (NOS) positive neuron expression in myenteric plexus of rat intestinal tract.
METHODS: Sprague Dawley rats were divided into experimental groups (portal triad clamping 20, 40, and 60 min group) and the control group. Then the specimens of intestinal myenteric plexus of small intestines and colons were collected and stained by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry.
RESULTS: In comparison with the control group, the numbers of NOS positive neurons of small intestinal (28.89 ± 5.49, 32.22 ± 7.03, 36.89 ± 8.58 vs 21.78 ± 4.56, P < 0.01) and colonic (34.22 ± 8.82, 39.39 ± 8.91, 44.61 ± 9.94 vs 25 ......
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