关键词: 血清素;肌，平滑;细胞，培养的;胃底;Ca2+ ;Fluo-3-AM;显微镜检查，共焦

    摘 要:目的 研究5-HT诱导胃底平滑肌细胞内钙动员的机制. 方法 采用Ca2+ 指示剂Fluo-3/AM作为细胞内钙离子的荧光探针负载培养的胃底平滑肌细胞，共聚焦技术检测细胞内钙荧光强度的变化. 结果 基础状态下SFSMC [Ca2+ ]i 荧光强度(FI)为264±15，5-HT10μmol L-1 使荧光强度的变化ΔFI为16.5±2.6;细胞外无钙时，5-HT升高[Ca2+ ]作用被减弱，但除去细胞外钙，并使用内罗啶(ryan-odine)孵育后，5-HT不再引起荧光强度的变化;10μmol L-1 米安舍林(mianserin)可部分拮抗5-HT升高[Ca2+ ]i 的作用，赛更定(cyprohetadine)不能抑制5-HT升高的胞内钙;Mn9202和拉西地平(lacidipine)均能完全抑制5-HT升高[Ca2+ ]i 的作用. 结论 5-HT可通过促进外钙内流及钙池(SR)释放使[Ca2+ ]i 升高;在胃底平滑肌5-HT升高胞内钙部分通过5-HT2 受体，而不是通过5-HT1C 受体;5-羟色胺通过L型钙通道促外钙内流;二氢吡啶类钙拮抗剂能通过阻滞L型钙通道，而完全抑制5-HT促胞内钙升高的作用.

    Mechanism of5┐hydroxytryptamine on introcellular calcium dynamics in stomach fundus smooth muscle cells of rats

    LIU Li-Hong，MEI Qi-Bing，CHEN Lei，JIA Min ，ZHAO Ming-Gao ，ZHAO De-Hua

    1 Department of Pharmacology，2 Department of His-tology and Embryology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:serotonin;muscle，smooth;cells，cultured;stomach fundus;Ca2+ ;Fluo-3/AM;mi-croscopy，confocal

    Abstract:AIM To study the mechanism of5-HT on[Ca2+ ]i dynamics in stomach fundus smooth muscle cells(SFSMC)of rats.METHODS Cells were cultured and loaded with Fluo-3/AM.[Ca2+ ]i was measured by fluores-cent intensity(FI)in each cell with confocal microscopy.RE┐SULTS Resting FI level of SFSMC was264±15 ......